In vivo effects of anti-asialo GM1. I. Reduction of NK activity and enhancement of transplanted tumor growth in nude mice.
In vivo effects of anti-asialo GM1. I. Reduction of NK activity and enhancement of transplanted tumor growth in nude mice.
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DOI：
10.4049/jimmunol.127.1.34
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发表时间：
1981-07
影响因子：
4.4
通讯作者：
S. Habu;H. Fukui;K. Shimamura;M. Kasai;Y. Nagai;K. Okumura;N. Tamaoki
中科院分区：
文献类型：
--
作者：
S. Habu;H. Fukui;K. Shimamura;M. Kasai;Y. Nagai;K. Okumura;N. Tamaoki
静脉注射抗asialo GM1在补体存在下能在体外消除自然杀伤(NK)活性，可完全消除裸鼠和常规小鼠脾细胞对淋巴瘤细胞株(YAC-1)的NK活性。免疫荧光研究显示，注射抗asialo GM1抗体的小鼠脾中asialo GM1阳性细胞的数量比注射正常兔血清的小鼠减少。在NK活性降低的裸鼠中，同基因肿瘤(RL Male-1)、同种异体肿瘤(YAC-1)和人肿瘤皮下移植后，肿瘤的成瘤率和生长均显著增加。这些结果有力地表明，NK细胞在体内移植瘤生长中起着重要作用。
Intravenous injection of anti-asialo GM1, which has been shown to eliminate natural killer (NK) activity in vitro in the presence of complement, completely abolished NK activity against lymphoma cell line (YAC-1) in spleen cells from athymic nude mice as well as from conventional mice. An immunofluorescence study revealed a decreased number of asialo GM1 positive cells in the spleens of mice injected with anti-asialo GM1 than in those of mice injected with normal rabbit serum. In the nude mice with reduced NK activity, incidence of tumor take and the growth were enhanced when syngeneic (RL male-1), and allogeneic (YAC-1) tumors and human tumors were transplanted subcutaneously. These results strongly suggest that NK cells play an important role in transplanted-tumor growth in vivo.