Re-emergence of Peste des Petits Ruminants virus in 2015 in Morocco: Molecular characterization and experimental infection in Alpine goats
Re-emergence of Peste des Petits Ruminants virus in 2015 in Morocco: Molecular characterization and experimental infection in Alpine goats
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DOI：
10.1016/j.vetmic.2016.11.006
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发表时间：
2016-12-25
影响因子：
3.3
通讯作者：
Elharrak, M.
中科院分区：
文献类型：
--
作者：
Fakri, F.;Embarki, T.;Elharrak, M.
小反刍兽疫（PPR）是一种小型反刍动物的跨界病毒性疾病，在非洲，中东和亚洲造成巨大的经济损失。在摩洛哥，2008年通报了第一次PPR疫情。此后7年没有报告病例，可能是由于2008-2011年期间连续三次疫苗接种运动和边境地区的密切监测。2015年6月，该疾病在摩洛哥重新出现，引发了人们对病毒起源的质疑。通过qRT-PCR确认PPR病毒，并在VeroNectin-4细胞上从临床样品中分离病毒。使用绵羊和山羊来源的爆发分离株在高山山羊中实验性复制了该疾病。2015年摩洛哥PPR分离株的分子表征证实该病毒为IV系，与2012年阿尔及利亚（KP 793696）和2012年突尼斯（KM 068121）分离株密切相关，与之前的PPRV摩洛哥2008株（HQ 131927）显著不同。因此，本研究证实了2015年期间PPR病毒在摩洛哥的新入侵，并强调了在北非马格里布地区实施共同控制战略以对抗PPR的紧迫性。(C)© 2016 Elsevier B. V.版权所有。
Peste des Petits Ruminants (PPR) is a transboundary viral disease of small ruminants that causes huge economic losses in Africa, The Middle East and Asia. In Morocco, the first PPR outbreak was notified in 2008. Since then no cases were reported for seven years, probably due to three successive vaccination campaigns during 2008-2011 and close surveillance at the border areas. In June 2015, the disease re-emerged in Morocco, raising questions about the origin of the virus. The PPR virus was confirmed by qRT-PCR and virus was isolated from clinical samples on VeroNectin-4 cells. The disease was experimentally reproduced in Alpine goats using both sheep and goat derived outbreak isolates. Molecular characterization of the 2015 Moroccan PPR isolate confirmed the identity of the virus as lineage IV, closely related to the 2012 Algerian (KP793696) and 2012 Tunisian (KM068121) isolates and significantly distinct from the previous PPRV Morocco 2008 strain (HQ131927). Therefore this study confirms a new incursion of PPR virus in Morocco during 2015 and highlights the urgency of implementation of a common control strategy to combat PPR in Maghreb region in North Africa. (C) 2016 Elsevier B.V. All rights reserved.