Isolation of UBP3, encoding a de-ubiquitinating enzyme, as a multicopy suppressor of a heat-shock mutant strain of S-cerevisiae
Isolation of UBP3, encoding a de-ubiquitinating enzyme, as a multicopy suppressor of a heat-shock mutant strain of S-cerevisiae
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DOI：
10.1007/s002940050354
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发表时间：
1998-06-01
期刊：
影响因子：
2.5
通讯作者：
Craig, EA
中科院分区：
文献类型：
--
作者：
Baxter, BK;Craig, EA
缺乏编码胞质分子伴侣的两个基因SSA1和SSA2的功能拷贝的酵母菌株对温度敏感。在这份报告中，我们描述了这种温度敏感性的高拷贝抑制因子UBP3的分离，它编码一种脱泛素化酶。我们发现ubp3突变酵母菌株具有轻微的缓慢生长表型，并积累泛素-蛋白质结合物。我们提出了一个模型，在该模型中，Ubp3p在体内发挥作用，逆转底物蛋白质的泛素化，使暂时错误折叠的蛋白质有机会正确折叠。
Yeast strains lacking functional copies of the two genes SSA1 and SSA2, which encode cytosolic molecular chaperones, are temperature-sensitive. In this report, we describe the isolation of a high-copy suppressor of this temperature sensitivity, UBP3, which encodes a de-ubiquitinating enzyme. We show that ubp3 mutant yeast strains have a mild slow-growth phenotype and accumulate ubiquitin-protein conjugates. We propose a model in which Ubp3p acts in vivo to reverse the ubiquitination of substrate proteins, allowing temporarily misfolded proteins an opportunity to fold correctly.