Tissue injury alters the site of expression of hepatocyte growth factor activator inhibitor type 1 in bronchial epithelial cells
Tissue injury alters the site of expression of hepatocyte growth factor activator inhibitor type 1 in bronchial epithelial cells
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DOI：
10.1111/j.1749-0774.2008.00062.x
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发表时间：
2009-02
期刊：
影响因子：
4.3
通讯作者：
Hiroyuki Tanaka;T. Fukushima;K. Yorita;M. Kawaguchi;H. Kataoka
中科院分区：
文献类型：
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作者：
Hiroyuki Tanaka;T. Fukushima;K. Yorita;M. Kawaguchi;H. Kataoka
肝细胞生长因子激活物抑制剂-1（HAI-1）是Kunitz型跨膜丝氨酸蛋白酶抑制剂，其抑制胰蛋白酶样丝氨酸蛋白酶，如肝细胞生长因子激活物、间质蛋白酶、肝蛋白酶和前列腺素。HAI-1在极化上皮细胞中表达，其中HAI-1主要位于基底外侧膜上。在本研究中，我们分析了HAI-1在呼吸道上皮中的表达和分布。我们发现，HAI-1表达的支气管呼吸上皮与基底或基底外侧定位，也由肺泡上皮。还使用培养的人支气管上皮细胞证实了HAI-1的支气管表达。HAI-1的上皮表达增加，以响应组织损伤，如癌症侵袭和炎症。令人惊讶的是，在受损的肺组织中，HAI-1在细支气管的纤毛上皮细胞中显示出明显的顶端易位。我们认为，除了其基底外侧表面定位，HAI-1可以短暂定位到呼吸纤毛上皮细胞的顶端表面严重炎症的条件下，可能与特定的细胞蛋白酶的顶端表面相互作用。
Hepatocyte growth factor activator inhibitor-1 (HAI-1) is a Kunitz-type transmembrane serine proteinase inhibitor that inhibits trypsin-like serine proteinases, such as hepatocyte growth factor activator, matriptase, hepsin and prostasin. HAI-1 is expressed in polarized epithelial cells, in which HAI-1 is mainly located on the basolateral membrane. In the present study, we analyzed the expression and distribution of HAI-1 in respiratory epithelium. We found that HAI-1 is expressed by the bronchial respiratory epithelium with basal or basolateral localization and also by the alveolar epithelium. Bronchial expression of HAI-1 was also confirmed using cultured human bronchial epithelial cells. The epithelial expression of HAI-1 was augmented in response to tissue injury such as cancer invasion and inflammation. Surprisingly, in the injured pulmonary tissue, HAI-1 showed distinct apical translocation in ciliated epithelial cells of the bronchiole. We suggest that, in addition to its basolateral surface localization, HAI-1 can transiently localize to the apical surface of respiratory ciliated epithelial cells under conditions of severe inflammation, possibly interacting with a specific cellular proteinase on the apical surface.