Repair of patellar tendon injuries using a cell-collagen composite
Repair of patellar tendon injuries using a cell-collagen composite
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DOI：
10.1016/s0736-0266(02)00163-8
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发表时间：
2003-05-01
影响因子：
2.8
通讯作者：
Butler, DL
中科院分区：
文献类型：
--
作者：
Awad, HA;Boivin, GP;Butler, DL
胶原凝胶接种兔骨髓间充质干细胞（MSC），并收缩到缝线上的初始细胞密度为1，4和8百万细胞/毫升。然后将这些MSC-胶原复合物植入在提供细胞的动物的髌腱中产生的全厚度、全长、中央缺损中。将这些自体修复与对侧相同缺损的自然修复进行比较。在术后6、12和26周对两种修复组织类型进行生物力学、组织学和形态学分析。在术后12周和26周，含有MSC-胶原复合物的修复组织显示出比天然修复组织显著更高的最大应力和模量。然而，在每个评价时间，不同接种密度的修复组织的任何尺寸或机械特性均未观察到显著差异。到26周，移植MSC-胶原复合物的修复是正常髌腱中心部分与骨端最大应力的四分之一。随着时间的推移，移植MSC-胶原复合物的修复组织的模量和最大应力的增加速度明显快于自然修复。出乎意料的是，28%的MSC-胶原移植肌腱在再生修复部位形成骨。除修复组织体积增加外，在自然修复和MSC-胶原移植修复之间未观察到细胞组织或组织学外观的显著差异。总的来说，这些结果表明，手术植入组织工程MSC-胶原复合材料显着改善肌腱修复组织的生物力学性能，虽然更大的MSC浓度没有产生额外的显着的组织学或生物力学改善。(C)2003骨科研究学会。由爱思唯尔科技有限公司出版。保留所有权利。
Collagen gels were seeded with rabbit bone marrow-derived mesenchymal stem cells (MSCs) and contracted onto sutures at initial cell densities of 1, 4, and 8 million cells/ml. These MSC-collagen composites were then implanted into full thickness, full length, central defects created in the patellar tendons of the animals providing the cells. These autologous repairs were compared to natural repair of identical defects on the contralateral side. Biomechanical, histological, and morphometric analyses were performed on both repair tissue types at 6, 12, and 26 weeks after surgery. Repair tissues containing the MSC-collagen composites showed significantly higher maximum stresses and moduli than natural repair tissues at 12 and 26 weeks postsurgery. However, no significant differences were observed in any dimensional or mechanical properties of the repair tissues across seeding densities at each evaluation time. By 26 weeks, the repairs grafted with MSC-collagen composites were one-fourth of the maximum stress of the normal central portion of the patellar tendon with bone ends. The modulus and maximum stress of the repair tissues grafted with MSC-collagen composites increased at significantly faster rates than did natural repairs over time. Unexpectedly, 28% of the MSC-collagen grafted tendons formed bone in the regenerating repair site. Except for increased repair tissue volume, no significant differences in cellular organization or histological appearance were observed between the natural repairs and MSC-collagen grafted repairs. Overall, these results show that surgically implanting tissue engineered MSC-collagen composites significantly improves the biomechanical properties of tendon repair tissues, although greater MSC concentrations produced no additional significant histological or biomechanical improvement. (C) 2003 Orthopaedic Research Society. Published by Elsevier Science Ltd. All rights reserved.