Optimization of mass transfer for toxin removal and immunoprotection of hepatocytes in a bioartificial liver.
Optimization of mass transfer for toxin removal and immunoprotection of hepatocytes in a bioartificial liver.
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DOI：
10.1002/bit.22450
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发表时间：
2009-12-01
影响因子：
3.8
通讯作者：
Nyberg, Scott L.
中科院分区：
文献类型：
--
作者：
Nedredal, Geir I.;Amiot, Bruce P.;Nyberg, Peter;Luebke-Wheeler, Jennifer;Lillegard, Joseph B.;McKenzie, Travis J.;Nyberg, Scott L.
本研究旨在确定生物人工肝（BAL）的最佳操作条件的基础上，代表性的肝毒素和免疫损伤介质的传质。一个微处理器控制的BAL被用来研究病人和细胞隔室之间的质量传递由中空纤维膜。在总共24种试验条件下，考虑了膜渗透性（70、150或400 kDa截留分子量-MWCO）、膜对流（高：50 mL/min;中：25 mL/min;低：10 mL/min;扩散：0 mL/min）和细胞室中的白蛋白浓度（0.5或5 g%）。最初，患者隔室含有补充有氨（0.017 kDa）、未结合胆红素（0.585 kDa）、结合胆红素（0.760 kDa）、TNF-α（17 kDa）、猪白蛋白（67 kDa）、猪IgG（147 kDa）和猪IgM（900 kDa）的猪血浆。每种物质的质量转移由其在细胞隔室中的出现率确定。葡聚糖聚合物技术评价膜污染。在三个测试变量（膜孔径、对流和白蛋白浓度）中，膜渗透性对传质的影响最大（P < 0.001）。所有毒素的传质是最大的高对流下与400 kDa的膜。IgG和IgM的转移在所有条件下均不显著。在高白蛋白条件下，胆红素转移增加（P = 0.055）。在2小时的测试间隔期间，膜的污染范围为7%（400 kDa）、24%（150 kDa）至62%（70 kDa）。总之，最佳的毒素去除下实现了高对流与400 kDa的膜，一个条件，这应该提供足够的免疫保护的肝细胞在BAL。
This study was designed to determine optimal operating conditions of a bioartificial liver (BAL) based on mass transfer of representative hepatotoxins and mediators of immune damage. A microprocessor-controlled BAL was used to study mass transfer between patient and cell compartments separated by a hollow fiber membrane. Membrane permeability (70, 150, or 400 kDa molecular weight cut-off—MWCO), membrane convection (high: 50 mL/min; medium: 25 mL/min; low: 10 mL/min; diffusion: 0 mL/min), and albumin concentration in the cell compartment (0.5 or 5 g%) were considered for a total of 24 test conditions. Initially, the patient compartment contained pig plasma supplemented with ammonia (0.017 kDa), unconjugated bilirubin (0.585 kDa), conjugated bilirubin (0.760 kDa), TNF-α (17 kDa), pig albumin (67 kDa), pig IgG (147 kDa), and pig IgM (900 kDa). Mass transfer of each substance was determined by its rate of appearance in the cell compartment. Membrane fouling was assessed by dextran polymer technique. Of the three tested variables (membrane pore size, convection, and albumin concentration), membrane permeability had the greatest impact on mass transfer (P < 0.001). Mass transfer of all toxins was greatest under high convection with a 400 kDa membrane. Transfer of IgG and IgM was insignificant under all conditions. Bilirubin transfer was increased under high albumin conditions (P = 0.055). Fouling of membranes ranged from 7% (400 kDa), 24% (150 kDa) to 62% (70 kDa) during a 2-h test interval. In conclusion, optimal toxin removal was achieved under high convection with a 400-kDa membrane, a condition which should provide adequate immunoprotection of hepatocytes in the BAL.
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影响因子：
3.8
作者：
Mavri-Damelin, Demetra;Damelin, Leonard H.;Hodgson, Humphrey J. F.
通讯作者：
Hodgson, Humphrey J. F.
影响因子：
6.2
作者：
Millis, JM;Cronin, DC;Maguire, P
通讯作者：
Maguire, P
影响因子：
4.6
作者：
Nyberg, SL;Hardin, J;Rinaldo, P
通讯作者：
Rinaldo, P
影响因子：
1.9
作者：
Patzer, J
通讯作者：
Patzer, J
影响因子：
4.2
作者：
Patzer, JF;Campbell, B;Miller, R
通讯作者：
Miller, R