Talin1 regulates the endometrial epithelial cell adhesive capacity by interacting with LASP1 and Vitronectin
Talin1 regulates the endometrial epithelial cell adhesive capacity by interacting with LASP1 and Vitronectin
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DOI：
10.1016/j.repbio.2020.02.006
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发表时间：
2020-06-01
影响因子：
2.1
通讯作者：
Qin, Aiping
中科院分区：
文献类型：
--
作者：
Li, Jingjing;Lin, Jiajing;Qin, Aiping
子宫内膜是一个高度复杂的组织，容易受到微妙的基因表达变化的影响，是植入胚泡的第一个接触点。Talin1已被发现可调节细胞骨架和细胞运动，但尚未发现与不孕症有关的研究。在此，我们提出Talin1基因异常在稽留流产子宫内膜中的表达异常会对子宫内膜的黏附能力产生负面影响。机制上，Talin1抑制LIM和SH3蛋白1(LASP1)的核转运，恢复黏附相关蛋白的表达。此外，细胞外Talin1通过与Vitronectin(VTN)相互作用，激活FAK/Src/ERK信号通路，增强子宫内膜上皮细胞的黏附能力。这一发现为Talin1在管理子宫内膜上皮细胞黏附方面的潜在应用提供了新的见解。这项研究首次证明Talin1在子宫内膜上皮细胞黏附和子宫内膜容受性中的作用。我们的研究结果表明，Talin1的重新表达可能是预防和治疗早期妊娠失败和不孕症的有用策略。
The endometrium is a highly complex tissue that is vulnerable to subtle gene expression changes and is the first point of contact for an implanting blastocyst. Talin1 has previously been identified to regulate cytoskeleton and cell motility, however it has not been investigated in association with infertility. Herein, we presented that Talin1 dysregulation in the missed abortion endometrium would negatively influence endometrial adhesive capacity. Mechanistically, intracellular Talin1 inhibited the nuclear transportation of LIM and SH3 protein 1 (LASP1) and restored the expression of adhesion-associated protein. Moreover, extracellular Talin1 enforces endometrial epithelial cell adhesive capacity by interacting with Vitronectin (VTN) and activating the FAK/Src/ERK signalling pathway. This finding provides a novel insight into the potential use of Talin1 for managing endometrial epithelia cell adhesion. This study represents the first demonstration of Talin1 function in endometrial epithelial cell adhesion and endometrial receptivity. Our findings indicate that re-expression of Talin1 might represent a useful strategy for preventing and treating early pregnancy failure and infertility.