Characterization in vivo and in vitro of a strain of Leishmania (Viannia) shawi from the Amazon Region
Characterization in vivo and in vitro of a strain of Leishmania (Viannia) shawi from the Amazon Region
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DOI：
10.1016/j.parint.2009.01.009
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发表时间：
2009-06-01
影响因子：
1.9
通讯作者：
de Souza, Wanderley
中科院分区：
文献类型：
--
作者：
Ramos, Patricia K. S.;Diniz, Jose A. P.;de Souza, Wanderley
The present study analyses complement resistance, cell surface carbohydrates expression, lipidic composition and morphology in vivo and in vitro, of Leishmania (Viannia) shawi, a parasite identified in the Amazon region, Para state, in 1989. We demonstrated that promastigotes in the stationary (STAT) growth phase are more resistant to complement lysis than in the logarithimic (LOG) growth phase. Ultrastructural analyses and imidazol technique showed accumulation of lipids in STAT growth phase promastigotes, which was confirmed by biochemical approach. Light and electron microscopy of skin lesion in hamster footpads caused by promastigotes in STAT growth phase, 90 days post inoculation, showed amastigotes inside of macrophage and free in the tissue Surrounded by collagen fibers as well as extensive inflammatory reaction with tissue destruction. We also demonstrated, using lectins by agglutination assays and How cytometry, the presence of fucose, mannose and/or glucose carbohydrate residues on the Surface of LOG and STAT promastigotes. The results constitute the first characterization essay combining biochemical and morphological approaches dedicated to LOG and STAT` growth phase promastigotes of L. (V) shawl contributing for a better knowledge of this poorly studied species of the New World. (C) 2009 Elsevier Ireland Ltd. All rights reserved.
