Structures of PmSOD1 and PmSOD2, two superoxide dismutases from the protozoan parasite Perkinsus marinus
Structures of PmSOD1 and PmSOD2, two superoxide dismutases from the protozoan parasite Perkinsus marinus
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DOI：
10.1107/s1744309106040425
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发表时间：
2006-11-01
影响因子：
0.9
通讯作者：
Silva, Abelardo M.
中科院分区：
文献类型：
--
作者：
Asojo, Oluwatoyin A.;Schott, Eric J.;Silva, Abelardo M.
Perkinsus marinus是东方牡蛎Crassostrea virgiica的一种兼性细胞内寄生虫，是造成牡蛎种群大量死亡的原因。海洋假单胞菌滋养体在牡蛎血细胞内存活和繁殖，侵入大多数组织和体液，从而引起全身感染，最终杀死宿主。海洋P.marinus滋养体的吞噬作用没有呼吸爆发，这表明这种寄生虫具有主动消除宿主氧化防御反应的机制。氧化损伤的一种机制和第一道防线是超氧化物歧化酶(SODS)将超氧阴离子自由基歧化为分子氧和过氧化氢。海洋假单胞菌具有两个铁相关的超氧化物歧化酶：PmSO1和PmSOD2。本文介绍了PmSOD_1和P_mSOD_2的晶化和X射线结构。
Perkinsus marinus, a facultative intracellular parasite of the eastern oyster Crassostrea virginica, is responsible for mass mortalities of oyster populations. P. marinus trophozoites survive and proliferate within oyster hemocytes, invading most tissues and fluids, thus causing a systemic infection that eventually kills the host. The phagocytosis of P. marinus trophozoites lacks a respiratory burst, suggesting that the parasite has mechanisms that actively abrogate the host's oxidative defense responses. One mechanism and the first line of defense against oxidative damage is the dismutation of superoxide radical to molecular oxygen and hydrogen peroxide by superoxide dismutases (SODs). P. marinus possesses two iron-cofactored SODs, PmSOD1 and PmSOD2. Here, the crystallization and X-ray structures of both PmSOD1 and PmSOD2 are presented.