RNA self-cleavage activated by ultraviolet light-induced oxidation
RNA self-cleavage activated by ultraviolet light-induced oxidation
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DOI：
10.1093/nar/gkr822
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发表时间：
2012-02-01
影响因子：
14.9
通讯作者：
Gomez, Jordi
中科院分区：
文献类型：
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作者：
Ariza-Mateos, Ascension;Prieto-Vega, Samuel;Gomez, Jordi
在丙型肝炎病毒（HCV）和相关的经典猪瘟病毒（CSFV）高度结构化的5'基因组区域中发现了一种新的uv -C光诱导的核酶活性。切割是由暴露于UV-C光介导的，而不是由外源性氧自由基介导的。它也具有很强的选择性，在一些分子中出现在HCV C-79和CSFV A（45）的碱基位置，在另一些分子中出现在毗邻的HCV U-78和CSFV U-44的5'位置。在其他反应产物中，检测到的大多数生物化学活性产物在新生成的末端含有3'-磷酸和5'-磷酸端基，同时3'-羟基端基的含量要低得多。虽然在切割位点附近保存E-loop RNA结构是HCV RNA自切割的必要条件，但CSFV RNA并非如此。与原始RNA基序兼容的反应性结构域（类似于33 nt）的短尺寸以及序列同源性的缺乏表明，尚未确定的紫外线激活核酶可能存在于整个结构RNA中，从而为早期RNA自裂事件是否由光敏RNA结构介导提供了线索。
A novel UV-C-light-induced ribozyme activity was discovered within the highly structured 5'-genomic regions of both Hepatitis C Virus (HCV) and the related Classic Swine Fever Virus (CSFV). Cleavage is mediated by exposure to UV-C light but not by exogenous oxygen radicals. It is also very selective, occurring at base positions HCV C-79 and CSFV A(45) in some molecules and at the immediately adjacent 5'-positions HCV U-78 and CSFV U-44 in others. Among other reaction products, the majority of biochemically active products detected contained 3'-phosphate and 5'-phosphate-end groups at the newly generated termini, along with a much lower amount of 3'-hydroxyl end group. While preservation of an E-loop RNA structure in the vicinity of the cleavage site was a requisite for HCV RNA self-cleavage, this was not the case for CSFV RNA. The short size of the reactive domains (similar to 33 nt), which are compatible with primitive RNA motifs, and the lack of sequence homology, indicate that as-yet unidentified UV-activated ribozymes are likely to be found throughout structured RNAs, thereby providing clues to whether early RNA self-cleavage events were mediated by photosensitive RNA structures.