Responsiveness of the human airway in vitro during deep inspiration and tidal oscillation
Responsiveness of the human airway in vitro during deep inspiration and tidal oscillation
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DOI：
10.1152/japplphysiol.01226.2010
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发表时间：
2011-06-01
影响因子：
3.3
通讯作者：
McFawn, Peter K.
中科院分区：
文献类型：
--
作者：
Noble, Peter B.;Jones, Robyn L.;McFawn, Peter K.
对于健康的人来说，深吸气会导致支气管扩张和呼吸道反应性降低，这可能是气道壁对机械拉伸的反应。这项研究的目的是检测离体人呼吸道对与正常呼吸相关的动态机械拉伸的体外反应。人体支气管段(n=6)取自肺肿瘤切除后无气流阻塞的患者。结扎侧支，并将气管安装在器官浴室中。在静态条件下和在有间歇性“深吸气”的“潮汐”振荡的情况下，测量气道狭窄至乙酰胆碱的累积浓度(3×10(-6)M至3×10(-3)M)。用马达控制的注射器泵(0.25赫兹潮气5~10cmH(2)O，深吸气5~30cmH(2)O)改变跨壁压力来模拟呼吸动作。通过管腔体积的减少来确定气道狭窄。潮气振荡对乙酰胆碱的气道反应没有影响，这与静态条件下的反应相似。在潮汐振荡、乙酰胆碱收缩的呼吸道中，深度吸气产生了强大的、短暂的(
In healthy individuals, deep inspiration produces bronchodilation and reduced airway responsiveness, which may be a response of the airway wall to mechanical stretch. The aim of this study was to examine the in vitro response of isolated human airways to the dynamic mechanical stretch associated with normal breathing. Human bronchial segments (n = 6) were acquired from patients without airflow obstruction undergoing lung resection for pulmonary neoplasms. The side branches were ligated and the airways were mounted in an organ bath chamber. Airway narrowing to cumulative concentrations of acetylcholine (3 x 10(-6) M to 3 x 10(-3) M) was measured under static conditions and in the presence of "tidal" oscillations with intermittent "deep inspiration." Respiratory maneuvers were simulated by varying transmural pressure using a motor-controlled syringe pump (tidal 5 to 10 cmH(2)O at 0.25 Hz, deep inspiration 5 to 30 cmH(2)O). Airway narrowing was determined from decreases in lumen volume. Tidal oscillation had no effect on airway responses to acetylcholine which was similar to those under static conditions. Deep inspiration in tidally oscillating, acetyl-choline-contracted airways produced potent, transient (