Proteomic analysis of Drosophila mojavensis male accessory glands suggests novel classes of seminal fluid proteins
Proteomic analysis of Drosophila mojavensis male accessory glands suggests novel classes of seminal fluid proteins
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DOI：
10.1016/j.ibmb.2009.03.003
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发表时间：
2009-05-01
影响因子：
3.8
通讯作者：
Markow, Therese A.
中科院分区：
文献类型：
--
作者：
Kelleher, Erin S.;Watts, Thomas D.;Markow, Therese A.
果蝇属的果蝇的特征在于受精性状的压倒性变化，如交配栓形成、精子储存器官的使用和营养射精捐赠。尽管对黑腹果蝇的遗传模型进行了广泛的研究，但对这些种间差异的分子基础知之甚少。本研究采用蛋白质组学的方法来精确定位莫哈韦果蝇（Drosophilamojavensis）的候选精液蛋白，莫哈韦果蝇是一种嗜恶果蝇，与莫哈韦果蝇相比具有不同的生殖生物学特性。黑腹菌我们确定了几类候选的精液蛋白质没有以前记录在D。黑腹雄性射精，包括代谢酶。营养转运蛋白和凝血因子。相反，我们还定义了29个在果蝇进化超过4000万年后仍然保守的SFP。我们讨论了我们的结果在昆虫繁殖的普遍过程，以及专门的生殖生物学的D。莫哈韦西斯。(C)2009爱思唯尔有限公司保留所有权利。
Fruit-flies of the genus Drosophila are characterized by overwhelming variation in fertilization traits such as copulatory plug formation, sperm storage organ use, and nutritional ejaculatory donation. Despite extensive research on the genetic model Drosophila melanogaster, little is known about the molecular underpinnings of these interspecific differences. This study employs a proteomic approach to pin-point candidate seminal fluid proteins in Drosophila mojavensis, a cactophilic fruit-fly that exhibits divergent reproductive biology when compared to D. melanogaster. We identify several classes of candidate seminal fluid proteins not previously documented in the D. melanogaster male ejaculate, including metabolic enzymes. nutrient transport proteins, and clotting factors. Conversely, we also define 29 SFPs that are conserved despite >40 million years of Drosophila evolution. We discuss our results in terms of universal processes in insect reproduction, as well as the specialized reproductive biology of D. mojavensis. (C) 2009 Elsevier Ltd. All rights reserved.