Curcumin attenuates murine lupus via inhibiting NLRP3 inflammasome
Curcumin attenuates murine lupus via inhibiting NLRP3 inflammasome
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姜黄素通过抑制 NLRP3 炎性体减轻小鼠狼疮
DOI：
10.1016/j.intimp.2019.01.046
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发表时间：
2019
影响因子：
5.6
通讯作者：
Tan Hechang
中科院分区：
文献类型：
--
作者：
Zhao Jijun;Wang Jihui;Zhou Mianjing;Li Mengyuan;Li Meirong;Tan Hechang
尽管对系统性红斑狼疮（SLE）的认识取得了快速进展，但仍然迫切需要新的更有效的干预措施。姜黄素是一种天然多酚化合物，已被证明在各种疾病中具有抗炎作用。在这项研究中，我们探讨了姜黄素在SLE中的潜在治疗价值。用姜黄素处理狼疮倾向雌性MRL/lpr小鼠。通过监测蛋白尿和组织学分析评估肾炎的发展和程度。采用酶联免疫吸附法测定血清抗dsDNA水平。通过Western印迹分析肾脏样品。在体外，在小鼠抗dsDNA抗体阳性（抗dsDNA+）血清存在下，使用小鼠足细胞进行研究。姜黄素治疗显著降低蛋白尿和肾脏炎症。姜黄素还降低了血清抗dsDNA水平和脾脏大小。此外，姜黄素减少了狼疮易感小鼠中的NLRP 3炎性小体激活。在体外，姜黄素显着抑制抗dsDNA+血清诱导的足细胞中NLRP 3炎性体的表达。总之，这些数据表明姜黄素在SLE治疗中的潜在用途。
Despite rapid progress in the understanding of systemic lupus erythematosus (SLE), there is still an urgent need for novel and more effective interventions. Curcumin, a natural polyphenol compound, has been shown to be anti-inflammatory in various disorders. In this study, we investigated the potential therapeutic value of curcumin in SLE. Lupus-prone female MRL/lpr mice were treated with curcumin. The development and extent of nephritis were assessed by monitoring proteinuria and by histologic analysis. Serum anti-dsDNA levels were measured by enzyme-linked immunosorbent assay. Kidney samples were analyzed by Western blot. In vitro, mouse podocytes were used for investigation in the presence of mouse anti-dsDNA antibody-positive (anti-dsDNA+) serum. Curcumin treatment dramatically decreased proteinuria and renal inflammation. Serum anti-dsDNA levels and spleen size were also reduced by curcumin. In addition, curcumin reduced NLRP3 inflammasome activation in lupus-prone mice. In vitro, curcumin significantly inhibited anti-dsDNA+ serum induced expression of NLRP3 inflammasome in podocytes. Overall, these data demonstrate the potential use of curcumin in SLE treatment.