THE SUPEROXIDE-GENERATING NADPH OXIDASE OF HUMAN-NEUTROPHILS IS ELECTROGENIC AND ASSOCIATED WITH AN H+ CHANNEL
THE SUPEROXIDE-GENERATING NADPH OXIDASE OF HUMAN-NEUTROPHILS IS ELECTROGENIC AND ASSOCIATED WITH AN H+ CHANNEL
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DOI：
10.1042/bj2460325
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发表时间：
1987-09-01
影响因子：
4.1
通讯作者：
JONES, OTG
中科院分区：
文献类型：
--
作者：
HENDERSON, LM;CHAPPELL, JB;JONES, OTG
来自人中性粒细胞的细胞质的膜电位被产生超氧化物的NADPH依赖的氧化酶激活而去极化。去极化的程度被二苯基碘离子抑制，因此直接归因于必须是产生电的氧化酶的活性。去极化的程度受到细胞膜上pH的变化的影响，这表明H+的向外转移最终补偿了超氧化物的产生。H+电流的阻断剂CD2+增强了电位的去极化，提示代偿性运动是通过H+通道实现的。
The membrane potential of cytoplasts, derived from human neutrophils, was depolarized by the activation of the superoxide-generating NADPH-dependent oxidase. The extent of the depolarization was inhibited by diphenylene iodonium and was therefore due directly to the activity of the oxidase, which must be electrogenic. The extent of the depolarization was influenced by alteration of the .DELTA.pH across the cytoplast membrane, indicating that the outward translocation of H+ eventually compensates for superoxide generation. The depolarization of the potential is enhanced by Cd2+, a blocker of H+ currents, suggesting that the compensatory movement is via an H+ channel.