Babesia: impact of cold storage on the survival and the viability of parasites in blood bags
Babesia: impact of cold storage on the survival and the viability of parasites in blood bags
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DOI：
10.1111/trf.12357
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发表时间：
2014-03-01
期刊：
影响因子：
2.9
通讯作者：
Lobo, Cheryl A.
中科院分区：
文献类型：
--
作者：
Cursino-Santos, Jeny R.;Alhassan, Andy;Lobo, Cheryl A.
巴贝斯虫是对血液供应的主要传染性威胁之一，因为临床无症状感染在人类中很常见，并且在某些接受者中可能危及生命。了解血液储存条件对巴贝斯虫生存能力的影响是很重要的，因为这将影响输血传播巴贝斯虫病的发生和严重程度。研究设计与方法将分化巴贝斯虫引入含有白细胞的血袋中，4℃保存0 ~ 31天。提取样本以评估寄生虫的存在、形态和生存能力。在不同的时间间隔从血袋中取出后立即进行血液涂片，并通过血膜显微镜进行评估。从袋中取出红细胞，在最适合寄生虫繁殖和生长的条件下培养8天，以评估寄生虫的生存能力。结果在4℃条件下保存24 h后，血袋内寄生虫率明显降低，并在整个保存过程中保持不变。这种减少伴随着寄生虫形态的变化，随着储存时间的推移，改变的寄生虫数量增加。然而，寄生物的生存能力在31天的冷藏中得以保持，在较长时间的冷藏中，寄生物在实现指数增长方面存在滞后。结论致病菌冷藏可引起寄生虫形态的改变和数量的减少。然而，有足够多的寄生虫足够健壮，可以在4摄氏度的条件下储存31天，并产生高终点寄生虫血症。
BackgroundBabesia represents one of the major infectious threats to the blood supply since clinically silent infections in humans are common and these can be life-threatening in certain recipients. It is important to understand the effect of blood storage conditions on the viability of Babesia as this will impact the occurrence and severity of transfusion-transmitted babesiosis.Study Design and MethodsBabesia divergens was introduced into blood bags containing leukoreduced red blood cells (RBCs) and stored at 4 degrees C for 0 to 31 days. Samples were withdrawn for assessment of the presence, morphology, and viability of parasites. Blood smears were made immediately on removal from blood bags at different time intervals and evaluated by blood film microscopy. RBCs withdrawn from the bags were also cultured for 8 days using conditions optimal for parasite reproduction and growth to allow assessment of parasite viability.ResultsAfter 24 hours of storage at 4 degrees C, there was a substantial reduction of parasitemia in the blood bags, which was maintained throughout storage. This decrease was accompanied by a change in morphology of parasites, with the number of altered parasites increasing through the period of storage. However, viability was maintained through 31 days of cold storage with a lag in achieving exponential growth seen in the parasites subjected to longer periods of refrigeration.ConclusionRefrigeration of B.divergens leads to an alteration of parasite morphology and a decrease in parasite numbers. However, there are sufficient parasites that are robust enough to survive 31 days of storage at 4 degrees C and yield high end-point parasitemia.