The carotenoid dioxygenase gene family in maize, sorghum, and rice.
The carotenoid dioxygenase gene family in maize, sorghum, and rice.
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DOI：
10.1016/j.abb.2010.07.019
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发表时间：
2010-12-01
影响因子：
3.9
通讯作者：
Wurtzel ET
中科院分区：
文献类型：
--
作者：
Vallabhaneni R;Bradbury LM;Wurtzel ET
类胡萝卜素及其类伪胡萝卜素衍生物具有重要的生理和发育作用，并提供植物对各种胁迫的耐受性。类胡萝卜素裂解双加氧酶介导类胡萝卜素降解为类胡萝卜素。更好地了解生物合成与降解可能有助于控制类胡萝卜素水平，从而提高植物适应性和/或提高有营养价值的类胡萝卜素含量。禾本科植物家族包含许多具有农艺价值的作物。因此，本研究的重点是鉴定禾本科植物玉米、水稻和高粱中的类胡萝卜素双加氧酶基因家族，并与两种非种子植物中新发现的基因家族以及一种藻类和先前发现的菊科植物基因进行比较。基因组分析用于定位编码类胡萝卜素双加氧酶的草基因的染色体位置。对编码蛋白序列进行系统发育比较。CCD8b是一类新的裂解双加氧酶，可能在类胡萝卜素的生物发生中起特殊作用。建立了一种简单的PCR方法来测定CCD1基因拷贝数，CCD1基因拷贝数在玉米中是不同的。利用一组类胡萝卜素含量不同的玉米自交系，线性回归分析显示，CCD1拷贝数与类胡萝卜素含量之间存在统计学上显著的负相关，这种效应可能是通过高拷贝数株系中胚乳CCD1转录物水平升高介导的。PCR检测增加了玉米胚乳类胡萝卜素代谢工程的增长工具箱。这个新工具可以用来选择不太可能促进胚乳类胡萝卜素降解的玉米品系，从而预测胚乳维生素原A和/或非维生素原A类胡萝卜素代谢工程的最佳结果。
Carotenoids and their apocarotenoid derivatives play essential physiological and developmental roles and provide plants tolerance to a variety of stresses. Carotenoid cleavage dioxygenases mediate the degradation of carotenoids to apocarotenoids. A better understanding of biosynthesis vs. degradation could be useful for controlling carotenoid levels leading to improved plant fitness and/or enhanced content of nutritionally valuable carotenoids. The Poaceae (grass) plant family contains many crops of agronomic value. Therefore this study focused on characterizing the carotenoid dioxygenase gene family in the grass species maize, rice, and sorghum with comparison made to newly identified gene families in two non-seed plants as well as an alga and previously identified eudicot genes. Genome analysis was used to map grass genes encoding the carotenoid dioxygenases to chromosome locations. Sequences of encoded proteins were phylogenetically compared. CCD8b was identified as a new class of cleavage dioxygenases that may play a specialized role in apocarotenoid biogenesis. A simple PCR assay was developed to measure CCD1 gene copy number which is known to vary in maize. Using a panel of maize inbred lines varying in carotenoid content, linear regression analysis revealed a statistically significant negative correlation between copy number of CCD1 and carotenoid content, an effect likely mediated through the resulting elevated levels of endosperm CCD1 transcripts in high copy number lines. The PCR assay adds to a growing toolbox for metabolic engineering of maize endosperm carotenoids. This new tool can be used to select maize lines that are less likely to promote endosperm carotenoid degradation, thus predicting optimal results in metabolic engineering of endosperm provitamin A and/or nonprovitamin A carotenoids.
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影响因子：
7.4
作者：
Li, Faqiang;Vallabhaneni, Ratnakar;Wurtzel, Eleanore T.
通讯作者：
Wurtzel, Eleanore T.
影响因子：
4.8
作者：
Kiefer, C;Hessel, S;von Lintig, J
通讯作者：
von Lintig, J
影响因子：
9.2
作者：
Booker, J;Auldridge, M;Leyser, O
通讯作者：
Leyser, O
影响因子：
7.4
作者：
Li, Faqiang;Murillo, Christina;Wurtzel, Eleanore T.
通讯作者：
Wurtzel, Eleanore T.
影响因子：
5.8
作者：
Kumar, S;Tamura, K;Nei, M
通讯作者：
Nei, M