Small Molecule Detection in Saliva Facilitates Portable Tests of Marijuana Abuse
Small Molecule Detection in Saliva Facilitates Portable Tests of Marijuana Abuse
复制标题
DOI：
10.1021/acs.analchem.6b01688
复制
发表时间：
2016-08-02
影响因子：
7.4
通讯作者：
Wang, Shan X.
中科院分区：
文献类型：
--
作者：
Lee, Jung-Rok;Choi, Joohong;Wang, Shan X.
随着医疗和娱乐使用大麻或大麻变得越来越普遍，执法部门需要一种工具来评估司机是否在大麻，特别是精神活性物质四氢大麻酚(THC)的影响下驾驶车辆。然而，导致损害的THC的截止浓度仍然存在争议，而且目前的现场筛查工具不够灵敏，无法检测到口腔液中的微量THC。在这里，我们提出了一种新型的传感平台，它使用巨磁阻(GMR)生物传感器与便携式阅读器系统和智能手机相集成，通过竞争性分析来检测唾液中的THC。通过一个简单的唾液收集方案，我们优化了测定THC的范围，范围从0到50 ng/mL，涵盖了以前研究中提出的大多数临界值。这项工作促进了对THC的现场筛查，并显示了在护理点(POC)环境中测试其他小分子药物和分析物的潜力。
As medical and recreational use of cannabis, or marijuana, becomes more prevalent, law enforcement needs a tool to evaluate whether drivers are operating vehicles under the influence of cannabis, specifically the psychoactive substance, tetrahydrocannabinol (THC). However, the cutoff concentration of THC that causes impairment is still controversial, and current on-site screening tools are not sensitive enough to detect trace amounts of THC in oral fluids. Here we present a novel sensing platform that employs giant magnetoresistive (GMR) biosensors integrated with a portable reader system and smartphone to detect THC in saliva using competitive assays. With a simple saliva collection scheme, we have optimized the assay to measure THC in the range from 0 to 50 ng/mL, covering most cutoff values proposed in previous studies. This work facilitates on-site screening for THC and shows potential for testing of other small molecule drugs and analytes in point-of-care (POC) settings.