Proteomic analysis in cardiovascular research.
Proteomic analysis in cardiovascular research.
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DOI：
10.1007/s00595-015-1169-4
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发表时间：
2016-03
期刊：
影响因子：
2.5
通讯作者：
Matsumoto K
中科院分区：
文献类型：
--
作者：
Oda T;Matsumoto K
使用临床人体样本的质谱技术和生物信息学的进步扩大了心血管研究中的定量蛋白质组学。有两种主要的蛋白质组学策略：即“基于凝胶”或“无凝胶”蛋白质组学与“自上而下”或“自下而上”质谱法相结合。两者都被引入蛋白质组学分析中，使用血浆或血清样本针对主动脉瘤和组织样本（例如动脉瘤壁、钙化主动脉瓣或心肌组织）的“生物标志物”搜索，研究病理生理学蛋白质相互作用和翻译后修饰。我们总结了分析心血管手术期间采集的人体样本以调查疾病过程的蛋白质组学研究，以便更好地了解已知分子因素和特定信号通路背后的全系统变化。
Advances in mass spectrometry technology and bioinformatics using clinical human samples have expanded quantitative proteomics in cardiovascular research. There are two major proteomic strategies: namely, “gel-based” or “gel-free” proteomics coupled with either “top-down” or “bottom-up” mass spectrometry. Both are introduced into the proteomic analysis using plasma or serum sample targeting ‘biomarker” searches of aortic aneurysm and tissue samples, such as from the aneurysmal wall, calcific aortic valve, or myocardial tissue, investigating pathophysiological protein interactions and post-translational modifications. We summarize the proteomic studies that analyzed human samples taken during cardiovascular surgery to investigate disease processes, in order to better understand the system-wide changes behind known molecular factors and specific signaling pathways.