Use of suppression subtractive hybridization to identify genetic differences between differentially virulent genotypes of Paenibacillus larvae, the etiological agent of American Foulbrood of honeybees
Use of suppression subtractive hybridization to identify genetic differences between differentially virulent genotypes of Paenibacillus larvae, the etiological agent of American Foulbrood of honeybees
复制标题
DOI：
10.1111/j.1758-2229.2009.00039.x
复制
发表时间：
2009-08-01
影响因子：
3.3
通讯作者：
Genersch, Elke
中科院分区：
文献类型：
--
作者：
Fuenfhaus, Anne;Ashiralieva, Ainura;Genersch, Elke
幼虫类芽孢杆菌（Paenibacillus larvae）是蜜蜂美洲幼虫病（American Foulbrood）的病原体，是一种致死性的蜜蜂幼虫病，对感染的蜂群也有致死性。最近，已经描述了四种不同的P.幼虫基因型（肠细菌重复基因间共有序列I-IV），并且显示这些基因型在表型上也不同，特别是在毒力上。为了解开这四种基因型之间的遗传差异，抑制消减杂交。从106个分析的克隆中，92个代表基因型特异性序列，而14个序列被证明仅对在减法中用作测试者的特定菌株具有特异性。近一半的序列（46%）只能根据表征不佳的序列进行注释。其余序列对应于与代谢相关的类别，特别是次级代谢物生物合成、运输和分解代谢、信息存储和处理以及细胞过程。特别是，我们可以证明幼虫疟原虫基因组包含编码抗生素的基因和/或巨大的基因簇，我们确定了第一个幼虫疟原虫毒素，腺苷二磷酸核糖基转移酶家族的成员。
Paenibacillus larvae is the causative agent of American Foulbrood of honeybees, a fatal brood disease not only killing infected larvae but also lethal to infected colonies. Recently four different genotypes of P. larvae (enterobacterial repetitive intergenic consensus I-IV) have been described and it was shown that these genotypes also differ in phenotype, especially in virulence. To unravel the genetic differences between these four genotypes, suppression subtractive hybridization was used. From 106 analysed clones, 92 represented genotype-specific sequences, whereas 14 sequences turned out to be specific only for the particular strain used as tester in the subtraction. Nearly half of the sequences (46%) could only be annotated based on poorly characterized sequences. The remaining sequences corresponded to categories related to metabolism, especially secondary metabolite biosynthesis, transport and catabolism, to information storage and processing, and to cellular processes. In particular, we could show that the P. larvae genome contains genes and/or giant gene clusters coding for antibiotics, and we identified the first P. larvae toxin, a member of the family of adenosine diphosphate-ribosyltransferases.