Fusion proteins containing neuropeptides as novel insect contol agents: snowdrop lectin delivers fused allatostatin to insect haemolymph following oral ingestion
Fusion proteins containing neuropeptides as novel insect contol agents: snowdrop lectin delivers fused allatostatin to insect haemolymph following oral ingestion
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DOI：
10.1016/s0965-1748(02)00105-4
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发表时间：
2002-12-01
影响因子：
3.8
通讯作者：
Edwards, JP
中科院分区：
文献类型：
--
作者：
Fitches, E;Audsley, N;Edwards, JP
雪花莲的甘露糖结合凝集素（雪花莲凝集素：GNA），当被喂食昆虫时，与肠道上皮细胞结合并进入血淋巴。通过在大肠杆菌中表达GNA/Manse-AS融合蛋白（FP）并将纯化的FP喂饲番茄蛾Lacanobia oleracea的幼虫，研究了GNA作为载体蛋白将昆虫神经肽Manse-AS递送至鳞翅目幼虫血淋巴的潜力。FP，在1.5%或0.5%的膳食蛋白质，被发现强烈抑制摄食和防止第五阶段幼虫的生长，而无论是GNA，也不是Manse-AS单独，也不是GNA和Manse-AS的混合物在对照处理，在类似的水平有有害影响。与抗Manse-AS抗体反应的材料水平升高，检测到在血淋巴中的昆虫喂养的饮食含有FP，这表明运输的肽发生。完整的FP的血淋巴的交付的证据提供了共同洗脱Manse AS样免疫反应性与标准FP后，从FP喂养的幼虫血淋巴的尺寸排阻色谱。GNA/Manse-AS和类似的融合蛋白提供了一种新的和有效的策略，通过口服给药昆虫神经肽，它可以用于在转基因植物中的表达，在田间给予作物保护。版权所有（C）2002由爱思唯尔科学有限公司出版。版权所有。
The mannose-binding lectin from snowdrop (Galanthus nivalis agglutinin: GNA), when fed to insects, binds to the gut epithelium and passes into the haemolymph. The potential for GNA to act as a carrier protein to deliver an insect neuropeptide, Manduca sexta allatostatin (Manse-AS), to the haemolymph of lepidopteran larvae has been examined by expressing a GNA/Manse-AS fusion protein (FP) in Escherichia coli, and feeding purified FP to larvae of the tomato moth Lacanobia oleracea. FP, administered at 1.5 or 0.5% of dietary proteins, was found to strongly inhibit feeding and prevent growth of fifth stadium larvae, whereas neither GNA nor Manse-AS alone, nor a mixture of GNA and Manse-AS in control treatments, had deleterious effects at similar levels. Elevated levels of material reacting with anti-Manse-AS antibodies were detected in the haemolymph of insects fed diets containing FP, suggesting that transport of the peptide had occurred. Evidence for the delivery of intact FP to the haemolymph was provided by the co-elution of Manse-AS-like immunoreactivity with standard FP after size exclusion chromatography of haemolymph from FP-fed larvae. GNA/Manse-AS and similar fusion proteins offer a novel and effective strategy for delivering insect neuropeptides by oral administration, which could be used in conjunction with expression in transgenic plants to give crop protection in the field. Crown copyright (C) 2002 Published by Elsevier Science Ltd. All rights reserved.