Cloning and expression analysis of follicle-stimulating hormone and luteinizing hormone receptor during the reproductive cycle in Korean rockfish (Sebastes schlegeli)
Cloning and expression analysis of follicle-stimulating hormone and luteinizing hormone receptor during the reproductive cycle in Korean rockfish (Sebastes schlegeli)
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DOI：
10.1007/s10695-012-9699-9
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发表时间：
2012-07
影响因子：
2.9
通讯作者：
W. Mu;H. Wen;F. He;Ji F. Li;Miao Liu;Y. Zhang;Jian Hu;B. Qi
中科院分区：
文献类型：
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作者：
W. Mu;H. Wen;F. He;Ji F. Li;Miao Liu;Y. Zhang;Jian Hu;B. Qi
使用逆转录聚合酶链反应 (PCR) 和 cDNA 末端快速扩增程序，从韩国石斑鱼 (Sebastes schlegeli) 的卵巢中分离出编码卵泡刺激素 (FSHR) 和黄体生成素 (LHR) 受体的全长 cDNA 序列。 KrFSHR 的 cDNA 编码一个由 703 个氨基酸组成的预测蛋白，与欧洲鲈鱼 (Dicentrarchus labrax) (78%) 和金头鲷 (Sparus aurata) (73%) 具有最大的同源性。 KrLHR 的 cDNA 编码预测的 703 个氨基酸的蛋白质，与欧洲鲈鱼 (Dicentrarchus labrax) (79%) 和金头鲷 (Sparus aurata) (76%) 表现出最高的同源性。除性腺外，在性腺外组织中也获得了 GTHRs mRNA 的表达。通过定量实时 PCR 检查了 KrGTHRs mRNA 性腺表达谱的季节性变化，目前的结果表明，KrFSHR mRNA 水平在性腺生长期间增加，而 KrLHR 在配子生成后期显示出高水平。我们的研究提供了 GTHR 的分子特征和生殖周期中的表达谱，强化了之前关于 GTHR 在韩国石斑鱼生殖内分泌调节中重要作用的知识。
Full-length cDNA sequences encoding the receptors for follicle-stimulating hormone (FSHR) and luteinizing hormone (LHR) were isolated from ovary of Korean rockfish (Sebastes schlegeli) using reverse transcription-polymerase chain reaction (PCR) and rapid amplification of cDNA ends procedures. The cDNA of the KrFSHR encodes a predicted protein of 703 amino acids that showed the greatest homology with European seabass (Dicentrarchus labrax) (78 %) and gilthead seabream (Sparus aurata) (73 %). The cDNA of the KrLHR encodes a predicted protein of 703 amino acids and exhibited the highest homology with European seabass (Dicentrarchus labrax) (79 %) and gilthead seabream (Sparus aurata) (76 %). Besides the gonads, expressions of GTHRs mRNA were also obtained in extra gonadal tissues. Seasonal changes in the gonads expression profiles of KrGTHRs mRNA were examined by quantitative real-time PCR, and the present results suggest that levels for KrFSHR mRNA increase during gonadal growth, whereas KrLHR shows high levels during the late gamete generation period. Our study provides molecular characterization of the GTHRs and expressions profile during reproductive cycles, reinforcing previous knowledge of GTHRs important role in the reproductive endocrine regulation of Korean rockfish.