Characterization of a novel Achromobacter xylosoxidans specific siphoviruse: phiAxp-1.
Characterization of a novel Achromobacter xylosoxidans specific siphoviruse: phiAxp-1.
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新型木糖氧化无色杆菌特异性虹病毒的表征：phiAxp-1
DOI：
10.1038/srep21943
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发表时间：
2016-02-24
影响因子：
4.6
通讯作者：
Zhao X
中科院分区：
文献类型：
--
作者：
Li E;Zhao J;Ma Y;Wei X;Li H;Lin W;Wang X;Li C;Shen Z;Zhao R;Jiang A;Yang H;Yuan J;Zhao X
由于木糖氧化无色杆菌的广泛存在，噬菌体最近被认为是一种替代的生物防治工具。本研究从中国渤海的水样中分离到一株特异性感染A.木糖氧化物。透射电子显微镜显示噬菌体phiAxp-1属于Siphoviridae。我们对phiAxp-1的基因组进行了测序，该基因组包含45，045 bp，具有64个开放阅读框。phiAxp-1编码的大多数蛋白质与公共数据库中的序列没有相似性。其中21个蛋白质与其它感染不同宿主的双链DNA噬菌体（如伯克霍尔德氏菌噬菌体KL 1、假单胞菌噬菌体73、假单胞菌噬菌体vB_E_Kakheti25、假单胞菌噬菌体vB_E_SCH_Ab26、不动杆菌噬菌体IME_AB3和无色杆菌噬菌体JWX）具有弱同源性。根据基因组的头尾结构、DNA复制和mazG的不同，基因组可以分为不同的簇。噬菌体phiAxp-1的序列和基因组结构明显不同于其他已知的Siphoviridae病毒，因此，我们建议它是Siphoviridae家族的一个新属的成员。对噬菌体的一步生长曲线和稳定性进行了研究，鉴定了phiAxp-1的特异性受体为A.木糖氧化物。
Bacteriophages have recently been considered as an alternative biocontrol tool because of the widespread occurrence of antimicrobial-resistant Achromobacter xylosoxidans. Herein, we isolated a virulent bacteriophage (phiAxp-1) from a water sample of the Bohai sea of China that specifically infects A. xylosoxidans. Transmission electron microscopy revealed that phage phiAxp-1 belongs to the Siphoviridae. We sequenced the genome of phiAxp-1, which comprises 45,045 bp with 64 open reading frames. Most of the proteins encoded by phiAxp-1 have no similarity to sequences in the public databases. Twenty-one proteins with assigned functions share weak homology with those of other dsDNA bacteriophages infecting diverse hosts, such as Burkholderia phage KL1, Pseudomonas phage 73, Pseudomonas phage vB_Pae-Kakheti25, Pseudomonas phage vB_PaeS_SCH_Ab26, Acinetobacter phage IME_AB3 and Achromobacter phage JWX. The genome can be divided into different clusters for the head and tail structure, DNA replication and mazG. The sequence and genomic organization of bacteriophage phiAxp-1 are clearly distinct from other known Siphoviridae phages; therefore, we propose that it is a member of a novel genus of the Siphoviridae family. Furthermore, one-step growth curve and stability studies of the phage were performed, and the specific receptor of phiAxp-1 was identified as the lipopolysaccharide of A. xylosoxidans.