MicroRNA Detection at Femtomolar Concentrations with Isothermal Amplification and a Biological Nanopore
MicroRNA Detection at Femtomolar Concentrations with Isothermal Amplification and a Biological Nanopore
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通过等温扩增和生物纳米孔检测飞摩尔浓度的 MicroRNA
DOI：
10.1007/978-1-0716-2982-6_5
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发表时间：
2023
影响因子：
--
通讯作者：
Kawano Ryuji
中科院分区：
文献类型：
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作者：
Takiguchi Sotaro;Kawano Ryuji
纳米孔传感是快速和无标记检测寡核苷酸（包括microRNA）的有力工具。当走向实际诊断应用时，低浓度microRNA的检测是需要解决的重要问题之一。我们在这里描述了一种使用等温扩增和纳米孔技术检测超低浓度microRNA的方法。使用这种方法，可以通过纳米孔测量来测量来自1 fM靶微小RNA的扩增DNA。
Nanopore sensing is a powerful tool for the rapid and label-free detection of oligonucleotides, including microRNA. When moving towards actual diagnostic applications, detection of microRNA at low concentrations is one of the significant issues to be addressed. We here describe a method to detect ultra-low concentrations of microRNA using isothermal amplification and nanopore technology. Using this method, the amplified DNA from 1 fM of target microRNA can be measured by a nanopore measurement.