Molecular characterization of a cDNA from the true armyworm Pseudaletia unipuncta encoding Manduca sexta allatotropin peptide(1).
Molecular characterization of a cDNA from the true armyworm Pseudaletia unipuncta encoding Manduca sexta allatotropin peptide(1).
复制标题
DOI：
10.1016/s0965-1748(00)00040-0
复制
发表时间：
2000-09
影响因子：
3.8
通讯作者：
P. Truesdell;P. Koladich;H. Kataoka;Kuniaki Kojima;A. Suzuki;J. N McNeil;A. Mizoguchi;S. Tobe;W. Bendena
中科院分区：
文献类型：
--
作者：
P. Truesdell;P. Koladich;H. Kataoka;Kuniaki Kojima;A. Suzuki;J. N McNeil;A. Mizoguchi;S. Tobe;W. Bendena
Allatotropin (AT) 是一种从烟草天蛾 Manduca sexta 中分离出来的昆虫神经肽，可刺激幼体的保幼激素 (JH) 生物合成。从真正的粘虫 Pseudaletia unipuncta 中分离出的 cDNA 编码一个 135 个氨基酸的 AT 前体肽，其中包含 AT 肽，以及其内切蛋白水解和酰胺化所需的加工位点，以及两个功能未知的附加肽。编码的 AT 肽与从 M. sexta 和 Agrius convolvuli 中分离的 AT 肽相同。 Southern印迹分析表明AT是每个单倍体基因组的单拷贝基因并且以两种等位基因形式存在。通过 Northern 印迹分析检测到大约 1.5 kb 的单个转录物。在从六龄幼虫到五日龄蛾的发育过程中分析了 AT 基因的表达。在蛹晚期观察到初始表达，并且这种表达在两性的整个成虫阶段都维持。在一天龄的蛾中，AT mRNA 的表达水平处于最低水平，但在第 3 天和第 5 天的成虫中水平有所增加。成年 P. unipuncta 蛾中 AT 的表达模式反映了 JH 生物合成的模式，并支持了 AT 可能在成虫阶段发挥作用的观点。免疫组织化学和原位杂交揭示 AT 表达定位于神经系统的许多结构，表明 AT 可能具有与 JH 生物合成调节不同的功能。
Allatotropin (AT) is an insect neuropeptide isolated from the tobacco hornworm, Manduca sexta, stimulates juvenile hormone (JH) biosynthesis by the corpora allata. A cDNA isolated from the true armyworm, Pseudaletia unipuncta, encodes a 135 amino acid AT precursor peptide which contains the AT peptide, with processing sites necessary for its endoproteolytic cleavage and amidation, plus two additional peptides of unknown function. The encoded AT peptide is identical to that isolated from M. sexta and Agrius convolvuli. Southern blot analysis indicated that AT is a single copy gene per haploid genome and is present in two allelic forms. A single transcript of approximately 1.5 kilobases was detected by northern blot analysis. The expression of the AT gene was analyzed during development from sixth instar larvae to five day-old moths. Initial expression was observed in late pupae and this expression was maintained throughout the adult stages in both sexes. In one day-old moths, expression was at its lowest level of the stages that express AT mRNA but levels increased in day 3 and day 5 adults. This pattern of AT expression in adult P. unipuncta moths mirrors that of JH biosynthesis and supports the notion that AT may act in the adult stages. Immunohistochemistry and in situ hybridization revealed that AT expression was localized to numerous structures of the nervous system, suggesting that AT may have functions distinct from regulation of JH biosynthesis.