Excretory/secretory products in the Echinococcus granulosus metacestode: is the intermediate host complacent with infection caused by the larval form of the parasite?
Excretory/secretory products in the Echinococcus granulosus metacestode: is the intermediate host complacent with infection caused by the larval form of the parasite?
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DOI：
10.1016/j.ijpara.2016.07.009
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发表时间：
2016-12-01
影响因子：
4
通讯作者：
Zaha, Arnaldo
中科院分区：
文献类型：
--
作者：
dos Santos, Guilherme B.;Monteiro, Karina M.;Zaha, Arnaldo
棘球绦虫属由寄生于两个哺乳动物宿主的寄生虫组成。它们的幼虫阶段称为包虫病囊，主要在中间寄主的肝脏和肺部发育。包虫囊肿是囊性包虫病的病原体，而G1单倍型的细粒棘球蚴（Echinococcus granulosus）是人类、牛羊中绝大多数病例的病因。包虫病蛋白的鉴定对更好地了解包虫病的宿主-寄生虫关系和包虫病的繁殖过程至关重要。本工作的目的是鉴定和定量比较颗粒棘球绦虫可育囊肿和不育囊肿包虫液中发现的蛋白质，以突出可能涉及囊肿生育或不育的机制。采用液相色谱-质谱联用技术（LC-MS/MS）对含细粒棘球蚴和牛棘球蚴蛋白的包虫液样品进行了分析。我们对可育囊肿和不育囊肿的蛋白质组学分析共鉴定出498种蛋白质，其中153种在可育囊肿中鉴定，271种在不育囊肿中鉴定，74种在两者中均鉴定。计算机功能分析使我们能够突出一些重要方面：(i)关于在可育和不育囊肿中涉及寄生虫和宿主反应的“军备竞赛”可能存在的线索；（ii）包虫液中一些没有功能注释或可能具有替代功能的蛋白质；（iii）细胞外囊泡如外泌体的存在，透射电镜证实了这一点。(C) 2016澳大利亚寄生虫学学会。Elsevier Ltd.出版。版权所有。
The genus Echinococcus consists of parasites that have a life cycle with two mammalian hosts. Their larval stage, called the hydatid cyst, develops predominantly in the liver and lungs of intermediate hosts. The hydatid cyst is the causative agent of cystic hydatid disease and the species Echinococcus granulosus, G1 haplotype, is responsible for the vast majority of cases in humans, cattle and sheep. Protein characterization in hydatid cysts is essential for better understanding of the host-parasite relationship and the fertility process of Echinococcus. The aims of this work were the identification and quantitative comparison of proteins found in hydatid fluid from fertile and infertile cysts from E. granulosus, in order to highlight possible mechanisms involved in cyst fertility or infertility. Hydatid fluid samples containing proteins from both E. granulosus and Bos taurus were analysed by LC-MS/MS. Our proteomic analysis of fertile and infertile cysts allowed identification of a total of 498 proteins, of which 153 proteins were exclusively identified in the fertile cyst, 271 in the infertile cyst, and 74 in both. Functional in silico analysis allowed us to highlight some important aspects: (i) clues about the possible existence of an "arms race" involving parasite and host responses in fertile and infertile cysts; (ii) a number of proteins in hydatid fluid without functional annotation or with possible alternative functions; (iii) the presence of extra cellular vesicles such as exosomes, which was confirmed by transmission electron microscopy. (C) 2016 Australian Society for Parasitology. Published by Elsevier Ltd. All rights reserved.