Dynamic control of head mesoderm patterning
Dynamic control of head mesoderm patterning
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DOI：
10.1242/dev.062737
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发表时间：
2011-07-01
期刊：
影响因子：
4.6
通讯作者：
Dietrich, Susanne
中科院分区：
文献类型：
--
作者：
Bothe, Ingo;Tenin, Gennadiy;Dietrich, Susanne
胚胎头部中胚层产生颅肌，并有助于头骨和心脏。在分化之前，通过分子标记的方式对组织进行区域化。我们发现，这种模式是建立在三个离散的阶段，都依赖于外在的线索。检测直接和第一波间接反应，我们发现，该过程是由动态组合以及拮抗作用的视黄酸（RA），BMP和FGF信号。在第1阶段，头部中胚层的初始前后（a-p）细分是响应于RA水平下降和FGF信号传导的激活而形成的。在第2阶段，BMP和Fgf信号增强a-p边界并改善前标记基因表达。在第3阶段，扩展FGF信号驱动MyoR和Tbx 1表达沿咽沿着的a-p扩展，RA限制MyoR的扩展。这建立了成熟的头部中胚层模式，具有区分预期的眼外和颌骨骼肌、鳃头肌和心脏流出道细胞的标记。
The embryonic head mesoderm gives rise to cranial muscle and contributes to the skull and heart. Prior to differentiation, the tissue is regionalised by the means of molecular markers. We show that this pattern is established in three discrete phases, all depending on extrinsic cues. Assaying for direct and first-wave indirect responses, we found that the process is controlled by dynamic combinatorial as well as antagonistic action of retinoic acid (RA), Bmp and Fgf signalling. In phase 1, the initial anteroposterior (a-p) subdivision of the head mesoderm is laid down in response to falling RA levels and activation of Fgf signalling. In phase 2, Bmp and Fgf signalling reinforce the a-p boundary and refine anterior marker gene expression. In phase 3, spreading Fgf signalling drives the a-p expansion of MyoR and Tbx1 expression along the pharynx, with RA limiting the expansion of MyoR. This establishes the mature head mesoderm pattern with markers distinguishing between the prospective extra-ocular and jaw skeletal muscles, the branchiomeric muscles and the cells for the outflow tract of the heart.