Fabrication and Characterization of Silk Microfiber-Reinforced Methacrylated Gelatin Hydrogel with Turnable Properties
Fabrication and Characterization of Silk Microfiber-Reinforced Methacrylated Gelatin Hydrogel with Turnable Properties
复制标题
具有可转动性能的丝微纤维增强甲基丙烯酸酯明胶水凝胶的制备和表征
DOI：
10.1080/09205063.2018.1493022
复制
发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Xiaoling Liao
中科院分区：
文献类型：
--
作者：
Wenqian Xiao;Yunfei Tan;Jiale Li;ChengFu Gu;Hang Li;Bo Li;Xiaoling Liao
尽管进行了大量的研究工作，但目前可用于组织工程的天然水凝胶存在几个主要缺点，其中一个重要问题是它们的机械强度差，无法满足成功结果的一些机械要求。在本文中，为了模拟天然细胞外基质的组成和结构，将通过碱水解获得的微米级丝纤维用作GelMA水凝胶中的增强相，导致材料具有比单独的纯GelMA水凝胶显著更大的刚度。此外，水凝胶表现出可调的压缩强度，溶胀能力，和降解性能的基础上的丝纤维长度。细胞实验表明，MC 3 T3-E1前成骨细胞迅速粘附并在复合水凝胶表面增殖，如FDA/PI染色和CCK-8测定所示。此外，各种细胞反应，包括细胞粘附，细胞形态和细胞增殖行为的变化，发生在复合水凝胶和不同的纤维长度。总的来说，这项研究介绍了一系列的纤维增强，可调复合水凝胶，可用于各种组织工程应用。
Despite considerable research effort, the natural hydrogels presently available for tissue engineering suffer from several major drawbacks, one of the significant issue is their poor mechanical strength which are unable to satisfy some mechanical requirements for successful outcomes. Herein, to mimic the composition and structure of the natural extracellular matrix, the micron-sized silk fibers obtained by alkaline hydrolysis were used as a reinforcement phase in a GelMA hydrogel, resulting in a material with significantly greater stiffness than pure GelMA hydrogel alone. In addition, the hydrogel demonstrated tunable compressive strength, swelling capacity, and degradation properties based on the silk fiber length. Experiments with cells indicated that MC3T3-E1 pre-osteoblasts quickly adhered to and proliferated on the surface of the composite hydrogels, as revealed by FDA/PI staining and CCK-8 assays. In addition, various cellular responses, including cell adhesion, changes in cellular morphology and cell proliferation behavior, occurred on the composite hydrogel and varied with fiber length. Overall, this study introduces a series of fiber-reinforced, tunable composite hydrogels that could be useful for various tissue engineering applications.