Structure and boosting activity of a starch-degrading lytic polysaccharide monooxygenase.
Structure and boosting activity of a starch-degrading lytic polysaccharide monooxygenase.
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DOI：
10.1038/ncomms6961
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发表时间：
2015-01-22
影响因子：
16.6
通讯作者：
Walton, Paul H.
中科院分区：
文献类型：
--
作者：
Lo Leggio, Leila;Simmons, Thomas J.;Poulsen, Jens-Christian N.;Frandsen, Kristian E. H.;Hemsworth, Glyn R.;Stringer, Mary A.;von Freiesleben, Pernille;Tovborg, Morten;Johansen, Katja S.;De Maria, Leonardo;Harris, Paul V.;Soong, Chee-Leong;Dupree, Paul;Tryfona, Theodora;Lenfant, Nicolas;Henrissat, Bernard;Davies, Gideon J.;Walton, Paul H.
裂解多糖单加氧酶（LPMO）是最近发现的氧化解构多糖的酶。 LPMO 对于细菌和真菌有效利用这些底物至关重要；此外，酶具有重要的工业重要性。我们在此报告了淀粉活性 LPMO（新 CAZy AA13 家族的代表）的活性、光谱和三维结构。我们证明这些酶从回生淀粉中产生醛糖酸封端的麦芽低聚糖，并显着促进β-淀粉酶将这种顽固底物转化为麦芽糖。酶活性位点的详细结构有助于深入了解这一类重要酶的作用机制。 裂解多糖单加氧酶 (LPMO) 是工业上重要的氧化解构多糖的酶。在这里，Lo Leggio 等人。报道了对顽固性回生淀粉具有活性的新 CAZy AA13 家族的 LPMO 的活性、光谱和三维结构。
Lytic polysaccharide monooxygenases (LPMOs) are recently discovered enzymes that oxidatively deconstruct polysaccharides. LPMOs are fundamental in the effective utilization of these substrates by bacteria and fungi; moreover, the enzymes have significant industrial importance. We report here the activity, spectroscopy and three-dimensional structure of a starch-active LPMO, a representative of the new CAZy AA13 family. We demonstrate that these enzymes generate aldonic acid-terminated malto-oligosaccharides from retrograded starch and boost significantly the conversion of this recalcitrant substrate to maltose by β-amylase. The detailed structure of the enzyme’s active site yields insights into the mechanism of action of this important class of enzymes. Lytic polysaccharide monooxygenases (LPMOs) are industrially important enzymes that oxidatively deconstruct polysaccharides. Here, Lo Leggio et al. report the activity, spectroscopy and three-dimensional structure of a LPMO of the new CAZy AA13 family active on recalcitrant-retrograded starch.
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影响因子：
64.8
作者：
通讯作者：
--
影响因子：
15
作者：
Hemsworth, Glyn R.;Taylor, Edward J.;Kim, Robbert Q.;Gregory, Rebecca C.;Lewis, Sally J.;Turkenburg, Johan P.;Parkin, Alison;Davies, Gideon J.;Walton, Paul H.
通讯作者：
Walton, Paul H.
DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
Emsley, P;Cowtan, K
通讯作者：
Cowtan, K
DOI：
10.1073/pnas.1323629111
发表时间：
2014-04-29
影响因子：
11.1
作者：
Agger, Jane W.;Isaksen, Trine;Westereng, Bjorge
通讯作者：
Westereng, Bjorge
DOI：
10.1107/s0907444909052925
发表时间：
2010-02
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
--
作者：
Adams PD;Afonine PV;Bunkóczi G;Chen VB;Davis IW;Echols N;Headd JJ;Hung LW;Kapral GJ;Grosse-Kunstleve RW;McCoy AJ;Moriarty NW;Oeffner R;Read RJ;Richardson DC;Richardson JS;Terwilliger TC;Zwart PH
通讯作者：
Zwart PH