Accurately quantifying the reductive capacity of microbial extracellular polymeric substance by mediated electrochemical oxidation method
Accurately quantifying the reductive capacity of microbial extracellular polymeric substance by mediated electrochemical oxidation method
复制标题
介导电化学氧化法准确定量微生物胞外聚合物的还原能力
DOI：
10.1016/j.scitotenv.2019.04.130
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发表时间：
2019
影响因子：
9.8
通讯作者：
Sheng Guo Ping
中科院分区：
文献类型：
--
作者：
Lin Zhi Qi;Shao Wei;Xu Juan;Sheng Guo Ping
The reductive capacity of microbial extracellular polymeric substances (EPS) plays important roles in environmental processes involved in heavy metal detoxification and organic contaminant degradation. However, the crucial parameter to evaluate the reductive capacity of EPS, electron donating capacity (EDC) lacks a quantitative approach. In this study, a novel mediated electrochemical oxidation (MEO) method was developed to investigate the EDCs of microbial EPS extracted fromShewanella oneidensisMR-1 (S. oneidensisMR-1),Escherichia coli(E. coli) and activated sludge. The results indicate that the MEO approach rapidly and accurately quantifies the EDCs of microbial EPS.S. oneidensisMR-1 EPS possessed the highest EDC value ascribed to their specific redox proteins components. EDCs ofS. oneidensisMR-1 EPS were dependent on measurement conditions and increased with growing solution pH and applied potential. EDCs ofS. oneidensisMR-1 EPS were depleted gradually during the redox reaction with irreversible oxidation of EPS. The reductive property of microbial EPS was accurately evaluated by quantifying the EDCs of EPS using the MEO approach, as well as their potential in environmental remediation.
