Genome-Wide Identification and Analysis of Genes, Conserved between japonica and indica Rice Cultivars, that Respond to Low-Temperature Stress at the Vegetative Growth Stage.
Genome-Wide Identification and Analysis of Genes, Conserved between japonica and indica Rice Cultivars, that Respond to Low-Temperature Stress at the Vegetative Growth Stage.
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DOI：
10.3389/fpls.2017.01120
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发表时间：
2017
影响因子：
5.6
通讯作者：
Kim SR
中科院分区：
文献类型：
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作者：
Kumar M;Gho YS;Jung KH;Kim SR
冷胁迫对农作物生产非常不利。然而，在水稻中只有几个基因被鉴定出与耐冷性相关的已知功能。为了更有效地应对这一农艺挑战，研究人员必须采取全球性的方法来选择有用的候选基因，并找到主要的调控因素。我们利用来自NCBI基因表达总集(http://www.ncbi.nlm.nih.gov/geo/)，)的冷胁迫处理样本产生的Affymetrix阵列数据的五个基因表达总括系列数据序列，鉴定了502个水稻品种和水稻品种共同的冷诱导基因。从这些结果中，我们证实了两个随机选择的基因在植物中受到冷胁迫后表达增加。此外，过表达OsWRKY71提高了供试品种‘东锦’的耐冷性。通过对水稻植株存活率和叶绿素荧光的计算，比较了两种水稻的耐寒性，证实了水稻的耐寒性更强。基因本体富集化分析表明，在冷胁迫条件下，生物过程中的‘L-苯丙氨酸分解代谢过程’的表达水平最高，这意味着它在水稻的这一反应中具有重要意义。MapMan分析将“主要代谢”过程和“调控基因模块”归类为冷应激反应的另外两个主要决定因素，并提出了几个关键的顺式调控元件。基于这些结果，我们提出了一个包含冷胁迫反应信号通路的模型。我们对该途径中确定的主要信号转导和转录调控因子的功能分析的结果将为我们深入了解新的调节代谢(S)，并为我们培育提高作物耐冷性的植物奠定基础。
Cold stress is very detrimental to crop production. However, only a few genes in rice have been identified with known functions related to cold tolerance. To meet this agronomic challenge more effectively, researchers must take global approaches to select useful candidate genes and find the major regulatory factors. We used five Gene expression omnibus series data series of Affymetrix array data, produced with cold stress-treated samples from the NCBI Gene Expression Omnibus (http://www.ncbi.nlm.nih.gov/geo/), and identified 502 cold-inducible genes common to both japonica and indica rice cultivars. From them, we confirmed that the expression of two randomly chosen genes was increased by cold stress in planta. In addition, overexpression of OsWRKY71 enhanced cold tolerance in ‘Dongjin,’ the tested japonica cultivar. Comparisons between japonica and indica rice, based on calculations of plant survival rates and chlorophyll fluorescence, confirmed that the japonica rice was more cold-tolerant. Gene Ontology enrichment analysis indicate that the ‘L-phenylalanine catabolic process,’ within the Biological Process category, was the most highly overrepresented under cold-stress conditions, implying its significance in that response in rice. MapMan analysis classified ‘Major Metabolic’ processes and ‘Regulatory Gene Modules’ as two other major determinants of the cold-stress response and suggested several key cis-regulatory elements. Based on these results, we proposed a model that includes a pathway for cold stress-responsive signaling. Results from our functional analysis of the main signal transduction and transcription regulation factors identified in that pathway will provide insight into novel regulatory metabolism(s), as well as a foundation by which we can develop crop plants with enhanced cold tolerance.
影响因子：
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作者：
E ZG;Wang L;Qin R;Shen H;Zhou J
通讯作者：
Zhou J