An epithelial cell line with elongated myoid morphology derived from bovine mammary gland. Expression of cytokeratins and desmosomal plaque proteins in unusual arrays.
An epithelial cell line with elongated myoid morphology derived from bovine mammary gland. Expression of cytokeratins and desmosomal plaque proteins in unusual arrays.
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DOI：
10.1016/0014-4827(83)90133-7
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发表时间：
1983-07
影响因子：
3.7
通讯作者：
E. Schmid;W. W. Franke-W.;C. Grund;D. Schiller;Heiderose Kolb;Neidhard Paweletz
中科院分区：
文献类型：
--
作者：
E. Schmid;W. W. Franke-W.;C. Grund;D. Schiller;Heiderose Kolb;Neidhard Paweletz
描述了选自牛乳腺上皮细胞培养物的克隆系（BMGE + HM）的细胞，其在达到汇合后不呈现典型的上皮样形态，而是形成细长的细胞，其细长的突起延伸到其它细胞的表面上。然而，显示非上皮样形态和异常丰富的肌动蛋白微丝的细胞系被确定为上皮细胞的细胞角蛋白和桥粒斑蛋白的合成，如通过免疫荧光和免疫电子显微镜和凝胶电泳的细胞骨架蛋白。细胞不产生波形蛋白和结蛋白丝。这些肌样细胞的特异性细胞角蛋白多肽与正常上皮样BMGE + H细胞中存在的那些相同，但排列在精细分散的非筋膜化细丝和颗粒结构的网状物的不寻常阵列中。桥粒斑蛋白，特别是桥粒斑蛋白，是丰富的，但桥粒的电子显微镜下的外观是异常的，因为它们中的大多数与第二个辅助斑形成在距离正常桥粒斑0.1-0.15 μm。细胞角蛋白丝和桥粒结构都存在于整个细胞质中，包括延伸的细胞突起。具有这种不寻常形态的上皮细胞系的存在证明了非形态学标准在鉴定上皮衍生细胞中的重要性。我们的研究结果还表明，上皮细胞的细胞形状和组织的巨大差异不一定与特定细胞骨架蛋白表达的变化有关。这种细胞系从肌上皮细胞的可能起源进行了讨论。
Cells of a clonal line (BMGE + HM) selected from bovine mammary gland epithelial cell cultures are described which, after reaching confluence, do not assume typical epithelioid morphology, but form elongated cells with long slender processes extending over the surfaces of other cells. However, cells of this line which display non-epithelioid morphology and are exceptionally rich in actin microfilaments are identified as epithelial cells by their synthesis of cytokeratins and desmosomal plaque proteins, as demonstrated by immunofluorescence and immunoelectron microscopy and by gel electrophoresis of cytoskeletal proteins. The cells do not produce vimentin and desmin filaments. The specific cytokeratin polypeptides of these myoid cells are identical to those present in normal epithelioid BMGE + H cells but are arranged in unusual arrays of meshworks of finely dispersed, non-fasciated filaments and granular structures. Desmosomal plaque proteins, notably desmoplakins, are abundant, but the electron microscopic appearance of the desmosomes is abnormal in that most of them are associated with a second accessory plaque formed at a distance of 0.1–0.15 μm from the normal desmosomal plaque. Both cytokeratin filaments and desmosomal structures are found throughout the whole cytoplasm, including the extended cell processes. The existence of an epithelial cell line with such an unusual morphology demonstrates the importance of non-morphological criteria in identifying epithelium-derived cells. Our findings also indicate that dramatic differences of cell shape and organization of epithelial cells need not necessarily be associated with changes in the expression of specific cytoskeletal proteins. The possible origin of this cell line from myoepithelial cells is discussed.