MHC Expression on Spleen Lymphocyte Subsets in Genetically Resistant and Susceptible Chickens Infected with Marek's Disease Virus
MHC Expression on Spleen Lymphocyte Subsets in Genetically Resistant and Susceptible Chickens Infected with Marek's Disease Virus
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DOI：
10.1089/vim.2009.0033
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发表时间：
2009-10-01
期刊：
影响因子：
2.2
通讯作者：
Juul-Madsen, Helle R.
中科院分区：
文献类型：
--
作者：
Dalgaard, Tina;Boving, Mette K.;Juul-Madsen, Helle R.
鸡的主要组织相容性复合体（MHC）对马立克病（MD）的抗性和易感性有很大的影响。本研究采用流式细胞术检测3种MHC基因型（B21/B21、B19/B21和B19/B19）耐药鸡和耐药鸡脾脏淋巴细胞的MHC表面表达情况。在脾脏中，组成型MHC I类表面表达在纯合子B19中最高，在纯合子B21中最低，在杂合子B19/B21中居中。这在脾淋巴细胞CD4（+）、CD8（+）和Bu-1（+）上观察到。将所有三种基因型的鸡分别感染MD病毒（GA株），并在感染后1、4和8周（p.i）分析被感染的鸡和mhc匹配阴性对照的脾脏样本。观察到MDV在感染后期诱导MHC I类表达增加。因此，与阴性对照相比，所有基因型感染鸡的CD4（+）细胞表面的MHC I类在4和8周p.i.时均有所增加。此外，除纯合子B19外，所有基因型感染鸡的CD8（+）细胞的MHC I类表达在第4周和第8周时均有所增加。mdv诱导的MHC II类表面水平也存在差异。因此，在感染B19/21和B21/B21的鸡的CD4（+）细胞上，MHC II类表达在第4周增加，在感染B19/B21的动物的CD4（+）细胞上，也在第8周增加。所有基因型感染鸡的CD8（+）细胞的MHC II类表达在第4周时均有所增加。这些发现表明，体内感染MDV增加了脾脏T细胞表面MHC的表达水平，这可能在免疫MDV中起作用。
Resistance and susceptibility to Marek's disease (MD) are strongly influenced by the chicken major histocompatibility complex (MHC). In this study, splenic lymphocytes from MD-resistant and MD-susceptible chickens of three MHC genotypes (B21/B21, B19/B21, and B19/B19) were analyzed by flow cytometry for MHC surface expression. In the spleen, constitutive MHC class I surface expression was found to be highest in homozygous B19, lowest in homozygous B21, and intermediate in heterozygous B19/B21 animals. This was observed on CD4(+), CD8(+), and Bu-1(+) splenic lymphocytes. Chickens of all three genotypes were subjected to infection with MD virus (GA strain) and spleen samples from infected as well as MHC-matched negative controls were analyzed at 1, 4, and 8 wk post-infection (p.i.). It was observed that MDV induced an increase in MHC class I expression late in the infection. Thus, MHC class I was increased on the surface of CD4(+) cells from infected chickens of all genotypes at 4 and 8 wk p.i. compared with negative controls. Also, MHC class I expression was increased on CD8(+) cells from infected chickens of all genotypes at 4 and 8 wk p.i., except for the homozygous B19 animals, that showed no increase at 8 wk p.i. MDV-induced differences in MHC class II surface levels were also found. Thus, MHC class II expression was increased on CD4(+) cells from infected B19/21 and B21/B21 chickens at 4 wk p.i., and also at 8 wk p.i. on CD4(+) cells from infected B19/B21 animals. MHC class II expression was increased on CD8(+) cells from infected chickens of all genotypes at 4 wk p.i. These findings suggest that MDV infection in vivo increases the level of MHC surface expression on splenic T cells, indicating a possible role in immunity against MDV.