Age related reference values for urine creatine and guanidinoacetic acid concentration in children and adolescents by gas chromatography-mass spectrometry
Age related reference values for urine creatine and guanidinoacetic acid concentration in children and adolescents by gas chromatography-mass spectrometry
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DOI：
10.1016/j.cccn.2004.05.013
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发表时间：
2004-10-01
影响因子：
5
通讯作者：
Vilarinho, L
中科院分区：
文献类型：
--
作者：
Valongo, C;Cardoso, ML;Vilarinho, L
一种用于常规定量尿液肌酸和胍乙酸 (GAA) 的新气相色谱-质谱法已被开发出来，以提供快速、可靠且廉价的代谢筛查。我们的方法采用两步衍生化程序，其中涉及与六氟乙酰丙酮的反应，然后与单三甲基三氟乙酰胺反应。标准曲线显示 GAA 的线性范围为 43-4269 mumol/l，肌酸的线性范围为 38-7325 mumol/l，涵盖了尿液中通常发现的 GAA 和肌酸的范围。 GAA的检测下限为1.54μmol/L，肌酸的检测下限为1.22μmol/L，GAA的检测下限为5.04μmol/L，肌酸的定量下限为4.19μmol/L。该方法还通过对169份尿液样本的分析，建立了健康婴儿、儿童和青少年中GAA和肌酸的参考值。虽然没有观察到性别差异，但正常的 GAA 尿水平和肌酸排泄量在年龄相关的亚组中是不同的。我们发现两个主要组的 GAA 存在统计学上显着的年龄差异（4 岁以下儿童，18-159 mumol/mmol 肌酐；5-16 岁受试者，18-130 mumol/mmol 肌酐），而三组肌酸存在差异（4 岁以下儿童，0.04-1.51 mmol/mmol 肌酐；5-11 岁受试者， 0.04-1.07 mmol/mmol 肌酐；12-16 岁受试者为 0.04-0.56 mmol/mmol 肌酐）。 (C) 2004 Elsevier B.V. 保留所有权利。
A new gas chromatography-mass spectrometry method for routine quantification of urine creatine and guanidinoacetic acid (GAA) has been developed to provide a fast, reliable and inexpensive metabolic screening. Our method uses a two-step derivatization procedure which involves a reaction with hexafluoroacetylacetone followed by a reaction with monotrimethylsityltrifluoroacetamide. The standard curves showed linearity over a range of 43-4269 mumol/l for GAA and 38-7325 mumol/l for creatine, which covers the range of GAA and creatine normally found in urine. The lower detection limit is 1.54 mumol/l for GAA and 1.22 mumol/l for creatine, whereas the lower quantification limit is 5.04 mumol/l for GAA and 4.19 mumol/l for creatine.This method was also employed to establish reference values for GAA and creatine in healthy infants, children and adolescents based on the analysis of 169 urine samples. Although no sex differences were observed, normal GAA urinary levels and creatine excretion are distinct in age-related subgroups. We identified a statistically significant age difference in two major groups for GAA (children under 4 years, 18-159 mumol/mmol creatinine; and subjects of 5-16 years, 18-130 mumol/mmol creatinine) whereas three groups were discriminated for creatine (children under 4 years, 0.04-1.51 mmol/mmol creatinine; subjects of 5-11 years, 0.04-1.07 mmol/mmol creatinine; and subjects of 12-16 years, 0.04-0.56 mmol/mmol creatinine). (C) 2004 Elsevier B.V. All rights reserved.