Crystallization and Preliminary X-ray Analysis of Dimeric and Trimeric Cytochromes c from Horse Heart
Crystallization and Preliminary X-ray Analysis of Dimeric and Trimeric Cytochromes c from Horse Heart
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马心二聚体和三聚体细胞色素 c 的结晶和初步 X 射线分析
DOI：
10.1107/s1744309110034913
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
S. Hirota and Y. Higuchi
中科院分区：
文献类型：
--
作者：
M. Taketa;H. Komori;Y. Hattori;S. Nagao;S. Hirota and Y. Higuchi
细胞色素c （cyt c）是线粒体呼吸链中的一种电子传递蛋白。已知它能形成聚合物，但其聚合机理尚不清楚。以聚乙二醇为沉淀剂，采用坐滴蒸汽扩散法成功地结晶了马的二聚体和三聚体细胞c。二聚体cyt c晶体属于空间群P1，单位细胞参数a = 41.8, b = 56.3, c = 60.8 Å， α = 66.3, β = 89.9, γ = 73.7°；三聚体cyt c晶体属于空间群P212121，单位细胞参数a = 57.2, b = 95.7, c = 130.9 Å。初始结构模型表明，二聚体和三聚体cytc晶体在不对称单元中分别含有两种二聚体和两种三聚体。
Cytochrome c (cyt c) is an electron-transfer protein in the respiratory chain of mitochondria. It is known to form polymers, but its polymerization mechanism is still unknown. Dimeric and trimeric cyt c from horse were successfully crystallized by the sitting-drop vapour-diffusion method using polyethylene glycol as a precipitating reagent. The crystal of dimeric cyt c belonged to space group P1, with unit-cell parameters a = 41.8, b = 56.3, c = 60.8 Å, α = 66.3, β = 89.9, γ = 73.7°, whereas that of trimeric cyt c belonged to space group P212121, with unit-cell parameters a = 57.2, b = 95.7, c = 130.9 Å. Initial structure models showed that the crystals of dimeric and trimeric cyt c contained two dimers and two trimers, respectively, in the asymmetric unit.