Expression and activity of p40MO15, the catalytic subunit of cdk-activating kinase, during Xenopus oogenesis and embryogenesis.
Expression and activity of p40MO15, the catalytic subunit of cdk-activating kinase, during Xenopus oogenesis and embryogenesis.
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p40MO15（cdk 激活激酶的催化亚基）在非洲爪蟾卵子发生和胚胎发生过程中的表达和活性。
DOI：
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发表时间：
1994
影响因子：
3.3
通讯作者：
J. Shuttleworth
中科院分区：
文献类型：
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作者：
A. Brown;T. Jones;J. Shuttleworth
cdk 激活激酶 (CAK) 对 p34cdc2 的苏氨酸 161 及其等效的 p33cdk2 中的苏氨酸 160 进行磷酸化对于这些细胞周期蛋白依赖性激酶的激活至关重要。我们研究了爪蟾卵子发生、减数分裂成熟和早期发育过程中 CAK 催化亚基 p40MO15 的表达和相关激酶活性，以更详细地了解 cdk 激酶在这些事件期间如何受到调节。我们发现 p40MO15 是一种稳定的蛋白质，半衰期 > 16 小时，在卵子发生过程中积累。 p40MO15 蛋白及其相关 CAK 活性主要位于生发囊泡；然而，在细胞质中发现了一小部分但重要的比例。在 VI 期卵母细胞中检测到的 p40MO15 量在减数分裂成熟、受精和早期胚胎发生过程中保持不变。值得注意的是，p40MO15被发现在卵子发生、减数分裂成熟和早期发育的快速有丝分裂周期期间具有组成型活性。这表明细胞周期进程中 p34cdc2 和 p33cdk2 活性的调节不涉及 p40MO15/CAK 水平或活性的变化。
Threonine 161 phosphorylation of p34cdc2 and its equivalent threonine 160 in p33cdk2 by cdk-activating kinase (CAK) is essential for the activation of these cyclin-dependent kinases. We have studied the expression and associated kinase activity of p40MO15, the catalytic subunit of CAK, during Xenopus oogenesis, meiotic maturation, and early development to understand in more detail how cdk kinases are regulated during these events. We find that p40MO15 is a stable protein with a half-life > 16 h that is accumulated during oogenesis. p40MO15 protein and its associated CAK activity are localized predominantly to the germinal vesicle; however, a small but significant proportion is found in the cytoplasm. The amount of p40MO15 detected in stage VI oocytes remains unchanged through meiotic maturation, fertilization, and early embryogenesis. Significantly, p40MO15 was found to be constitutively active during oogenesis, meiotic maturation, and the rapid mitotic cycles of early development. This suggests that regulation of p34cdc2 and p33cdk2 activity during cell cycle progression does not involve changes in the level or activity of p40MO15/CAK.
影响因子：
3.3
作者：
DESAI, D;GU, Y;MORGAN, DO
通讯作者：
MORGAN, DO
影响因子：
3.3
作者：
CONNELLCROWLEY, L;SOLOMON, MJ;HARPER, JW
通讯作者：
HARPER, JW