Overexpression of rice OsREX1-S, encoding a putative component of the core general transcription and DNA repair factor IIH, renders plant cells tolerant to cadmium- and UV-induced damage by enhancing DNA excision repair
Overexpression of rice OsREX1-S, encoding a putative component of the core general transcription and DNA repair factor IIH, renders plant cells tolerant to cadmium- and UV-induced damage by enhancing DNA excision repair
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DOI：
10.1007/s00425-014-2042-1
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发表时间：
2014-05-01
期刊：
影响因子：
4.3
通讯作者：
Kusano, Tomonobu
中科院分区：
文献类型：
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作者：
Kunihiro, Shuta;Kowata, Hikaru;Kusano, Tomonobu
从40,000个表达水稻全长cDNA的拟南芥Fox(Full-Long-cDNA Over-eXpressor gene Hunting System)系中筛选出4个耐镉(Cd)品系，其中一个转基因品系携带OsREX1-S。OsREX1-S与莱茵衣藻Rex1-S(简称CrREX1-S，其中REX表示切除所需)以及酵母和人TFB5(RNA聚合酶II转录因子B5)的同源性最高，这两种基因都是通用转录和DNA修复因子TFIIH的组成部分。OsREX1-S的瞬时表达一致地将该蛋白定位于洋葱细胞核。新获得的表达OsREX1-S的转基因拟南芥植株对Cd的耐性显著增强，证实了最初鉴定的品系的Cd耐性仅由OsREX1-S的表达所决定。此外，转OsREX1-S基因的拟南芥植株通过减少UVB辐射产生的环丁烷嘧啶二聚体的量而表现出对UVB的耐性。此外，与野生型相比，转OsREX1-S基因拟南芥植株对博莱霉素(DNA链断裂诱导剂)和丝裂霉素C(DNA插层活性)产生了抗性。我们的结果表明，OsREX1-S通过增强DNA切割修复使寄主植物对Cd、UVB辐射、博莱霉素和丝裂霉素C具有耐受性。
Screening of 40,000 Arabidopsis FOX (Full-length cDNA Over-eXpressor gene hunting system) lines expressing rice full-length cDNAs brings us to identify four cadmium (Cd)-tolerant lines, one of which carried OsREX1-S as a transgene. OsREX1-S shows the highest levels of identity to Chlamydomonas reinhardtii REX1-S (referred to as CrREX1-S, in which REX denotes Required for Excision) and to yeast and human TFB5s (RNA polymerase II transcription factor B5), both of which are components of the general transcription and DNA repair factor, TFIIH. Transient expression of OsREX1-S consistently localized the protein to the nucleus of onion cells. The newly generated transgenic Arabidopsis plants expressing OsREX1-S reproducibly displayed enhanced Cd tolerance, confirming that the Cd-tolerance of the initial identified line was conferred solely by OsREX1-S expression. Furthermore, transgenic Arabidopsis plants expressing OsREX1-S exhibited ultraviolet-B (UVB) tolerance by reducing the amounts of cyclobutane pyrimidine dimers produced by UVB radiation. Moreover, those transgenic OsREX1-S Arabidopsis plants became resistant to bleomycin (an inducer of DNA strand break) and mitomycin C (DNA intercalating activity), compared to wild type. Our results indicate that OsREX1-S renders host plants tolerant to Cd, UVB radiation, bleomycin and mitomycin C through the enhanced DNA excision repair.