Assessing the early changes of cerebral glucose metabolism via dynamic 18FDG-PET/CT during cardiac arrest
Assessing the early changes of cerebral glucose metabolism via dynamic 18FDG-PET/CT during cardiac arrest
复制标题
通过动态(18)FDG-PET/CT评估心脏骤停期间脑葡萄糖代谢的早期变化
DOI：
10.1007/s11011-015-9658-0
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发表时间：
2015-08-01
影响因子：
3.6
通讯作者：
Li, Xin
中科院分区：
文献类型：
--
作者：
Li, Ying-Qing;Liao, Xiao-Xing;Li, Xin
为研究心脏骤停(CA)后自主循环(ROSC)复苏期脑葡萄糖代谢(CGM)的变化，采用18F-脱氧葡萄糖正电子发射计算机断层扫描(18FDG-PET/CT)对6只Beagle犬模型进行了脑葡萄糖代谢的测定。记录基线(18)FDG-PET/CT后，在30min内诱发室颤(VF)，然后闭胸心肺复苏(CPR)，同时静脉注射肾上腺素和体外除颤器电击，直到达到ROSC。分别于静脉给药前、给药前、给药后4、24、48h记录18FDG。在上述四个时间点，我们用免疫组织化学方法检测了两个关键调控因子己糖激酶I(HXK I)和HXK II在犬CGM中的表达。电诱导性室性心动过速可持续6min。然后进行复苏以维持血压稳定。连续18次FDG-PET/CT扫描发现，CGM在ROSC后4h开始下降，48h仍低于基线水平，HXK I和II的表达水平与CGM的变化一致。这些数据表明，(18)FDG-PET/CT显像可用于检测CA期间CGM的减少，并与CMRg1的结果一致。此外，HXK I和HXK II的表达也发生了相应的变化。CGM的减少可能是超急性全脑缺血的早期征象。
To study the changes of cerebral glucose metabolism (CGM) during the phase of return of spontaneous circulation (ROSC) after cardiac arrest (CA), we used 18-fluorodeoxyglucose-positron emission tomography/computed tomography ((18)FDG-PET/CT) to measure the CGM changes in six beagle canine models. After the baseline (18)FDG-PET/CT was recorded, ventricular fibrillation (VF) was induced for 6 min, followed by close-chest cardiopulmonary resuscitation (CPR) in conjunction with intravenous (IV) administration of epinephrine and external defibrillator shocks until ROSC was achieved, within 30 min. The (18)FDG was recorded prior to intravenous administration at 0 h (baseline), and at 4, 24, and 48 h after CA with ROSC. We evaluated the expression of two key control factors in canine CGM, hexokinase I (HXK I) and HXK II, by immunohistochemistry at the four above mentioned time points. Electrically induced VF of 6 min duration was successfully induced in the dogs. Resuscitation was then performed to maintain blood pressure stability. Serial (18)FDG-PET/CT scans found that the CGM decreased at 4 h after ROSC and remained lower than the baseline even at 48 h. The expression of HXK I and II levels were consistent with the changes in CGM. These data from our present work showed that (18)FDG-PET/CT imaging can be used to detect decreased CGM during CA and was consistent with the results of CMRgl. Furthermore, there were also concomitant changes in the expression of HXK I and HXK II. The decrease in CGM may be an early sign of hyperacute global cerebral ischemia.