Bacterioplanoides pacificum gen. nov., sp. nov., isolated from seawater of South Pacific Gyre.
Bacterioplanoides pacificum gen. nov., sp. nov., isolated from seawater of South Pacific Gyre.
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太平洋拟杆菌属
DOI：
10.1099/ijsem.0.001461
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发表时间：
2016
影响因子：
2.8
通讯作者：
Xiaohua Zhang
中科院分区：
文献类型：
--
作者：
Yanan Wang;Mingchao Yu;Yan Liu;Xiaoting Yang;Xiaohua Zhang
在综合大洋钻探计划第329次考察期间，从南太平洋环流表层海水中分离到一株革兰氏染色阴性、严格好氧、透明光滑、弯曲杆状、具有单极鞭毛的能动细菌，命名为sw 100 T。基于16 S rRNA基因序列的系统发育分析表明，菌株sw 100 T与海洋浮游菌科的成员最接近，并且与最接近的属Bacterioplanes、Thalassolituus和Oceanalus不同（16 S rRNA基因序列相似性分别为95.8%、94.5%-95.3%和94.4%）。在0- 11%NaCl（w/v，最佳7%）存在下，在10-37 °C（最佳28 °C）和pH 6.0-9.0（最佳pH 7.0）下发生生长。主要脂肪酸（>总脂肪酸的10%）是C16：0、C16：0 N醇和总和特征3（C16：1ω6c和/或C16：1ω 7 c）。主要极性脂质为磷脂酰乙醇胺、磷脂酰单甲基乙醇胺、磷脂酰甘油、双磷脂酰甘油和两种未鉴定的极性脂质。菌株sw 100 T的DNA G+C含量为55.3mol%。主要呼吸醌是泛醌-10（Q-10）。基于多相分析，菌株sw 100 T被认为代表海洋浮游菌科新属中的新种，其名称太平洋拟杆菌属，sp. nov.的报道。模式菌株为sw 100 T（=JCM 30597 T =MCCC 1 K 00501 T =KCTC 42424 T）。
A Gram-staining-negative, strictly aerobic, transparent and smooth, curved-rod-shaped motile bacterium with a single polar flagellum, designated strain sw100T, was isolated from surface seawater of South Pacific Gyre during Integrated Ocean Drilling Program Expedition 329. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain sw100T was most closely related to the member of the family Oceanospirillaceae and was distinct from the most closely related genera Bacterioplanes, Thalassolituus and Oceanobacter (95.8, 94.5-95.3 and 94.4 % 16S rRNA gene sequence similarities, respectively). Growth occurred in the presence of 0-11 % NaCl (w/v, optimum 7 %), at 10-37 °C (optimum 28 °C) and pH 6.0-9.0 (optimum pH 7.0). The major fatty acids (>10 % of total fatty acids) are C16 : 0, C16 : 0 N alcohol and summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c). The major polar lipids were phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and two unidentified polar lipids. The DNA G+C content of strain sw100T was 55.3 mol%. The major respiratory quinone was ubiquinone-10 (Q-10). On the basis of polyphasic analyses, strain sw100T is considered to represent a novel species in a novel genus of the family Oceanospirillaceae, for which the name Bacterioplanoides pacificum gen. nov., sp. nov. is proposed. The type strain is sw100T (=JCM 30597T=MCCC 1K00501T=KCTC 42424T).
DOI：
10.1099/ijs.0.038075-0
发表时间：
2012-03-01
影响因子：
2.8
作者：
Kim, Ok-Sun;Cho, Yong-Joon;Chun, Jongsik
通讯作者：
Chun, Jongsik