Platelet-rich plasma diminishes calvarial bone repair associated with alterations in collagen matrix composition and elevated CD34+cell prevalence
Platelet-rich plasma diminishes calvarial bone repair associated with alterations in collagen matrix composition and elevated CD34+cell prevalence
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DOI：
10.1016/j.bone.2010.02.026
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发表时间：
2010-06-01
期刊：
影响因子：
4.1
通讯作者：
Urban, Cicero de Andrade
中科院分区：
文献类型：
--
作者：
Giovanini, Allan Fernando;Deliberador, Tatiana Miranda;Urban, Cicero de Andrade
血小板与 I 型和 III 型胶原之间的相互作用在调节血小板粘附和聚集方面发挥着重要作用，也有助于 CD34+ 细胞的趋化性。使用 PRP 时，与 III 型胶原蛋白的相互作用可以维持高水平的胶原蛋白并改变骨修复的生物学特性。本研究的目的是评估富血小板血浆 (PRP) 和自体移植物对 III 型和 I 型胶原的存在、它们之间的比例以及 CD34+ 祖细胞的存在的影响，同时通过骨组织的组织形态计量分析来比较这些结果。 23只兔子的颅骨上产生4处骨缺损（直径8.0毫米，深度2.0毫米）。手术缺损可采用自体骨移植、自体骨移植加 PRP 和单独 PRP 进行治疗。手术后2、4或6周对动物实施安乐死。进行组织学、组织形态计量学和免疫组织化学分析以评估修复时间、I 型和 III 型胶原蛋白的表达以及祖细胞 CD34+ 细胞的数量。使用方差分析和 Student-Newman-Keuls 检验（α= 5%）对数据进行分析。在 PRP 组中观察到肉芽和髓质组织区域增大。本研究中 PRP 的使用阻碍了骨沉积，还增强了 III 型与 I 型胶原蛋白的比例以及 CD34+ 祖细胞的趋化性，类似于血栓形成效应。 (C) 2010 Elsevier Inc. 保留所有权利。
The interaction between platelets and both type I and Ill collagens plays an important role in modulating platelet adhesion and aggregation, also contributing to the chemotaxis of CD34+ cells. The interaction with type III collagen can maintain high levels of collagen and alter the biology of hone repair when the PRP is used. The aim of this study was to evaluate the effect of platelet-rich plasma (PRP) and autograft on the presence of type Ill and type I collagens, the ratio between them, as well as the presence of CD34+ progenitor cells, while comparing these results by means of a histomorphometric analysis of the bone tissue. Four bone defects (8.0 mm in diameter and 2.0 mm in depth) were produced on the calvarium of 23 rabbits. The surgical defects were treated with either autogenous bone grafts, autogenous bone grafts with PRP and PRP alone. Animals were euthanized at 2, 4 or 6 weeks post-surgery. Histological, histomorphometric and immunohistochemical analyses were performed to assess repair time, as well as the expression of type I and III collagens, and number of progenitor CD34+ cells. Data were analyzed using the ANOVA and Student-Newman-Keuls test (alpha= 5%). An enlarged granulation and medullary tissue areas in the PRP groups were observed. The use of PRP in this study hindered bone deposition, also enhanced type III to type I collagen ratio and the chemotaxis of CD34+ progenitor cells, similarly to a thrombogenic effect. (C) 2010 Elsevier Inc. All rights reserved.