Rab6 regulates transport and targeting of exocytotic carriers
Rab6 regulates transport and targeting of exocytotic carriers
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DOI：
10.1016/j.devcel.2007.06.010
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发表时间：
2007-08-01
影响因子：
11.8
通讯作者：
Akhmanova, Anna
中科院分区：
文献类型：
--
作者：
Grigoriev, Ilya;Splinter, Daniel;Akhmanova, Anna
组成型胞吐作用将新合成的蛋白质、脂质和其他分子从高尔基体递送到细胞表面。这一过程是由小泡介导的，小泡从高尔基体网络上出芽，沿着细胞骨架丝移动，并与质膜融合。在这里，我们表明，小GTTRab 6标记胞吐囊泡，并与微管加末端定向运动驱动蛋白-1，刺激他们的进行性微管为基础的运输到细胞周边。此外，Rab 6引导分泌囊泡靶向富含皮质蛋白ELKS（一种已知的Rab 6结合伴侣）的质膜位点。我们的数据表明，虽然Rab 6是不是必不可少的分泌，它控制的组织胞吐在细胞空间。
Constitutive exocytosis delivers newly synthesized proteins, lipids, and other molecules from the Golgi apparatus to the cell surface. This process is mediated by vesicles, which bud off the trans-Golgi network, move along cytoskeletal filaments, and fuse with the plasma membrane. Here, we show that the small GTPase Rab6 marks exocytotic vesicles and, together with the microtubule plus-enddirected motor kinesin-1, stimulates their processive microtubule-based transport to the cell periphery. Furthermore, Rab6 directs targeting of secretory vesicles to plasma-membrane sites enriched in the cortical protein ELKS, a known Rab6 binding partner. Our data demonstrate that although Rab6 is not essential for secretion, it controls the organization of exocytosis within the cellular space.