GENE-EXPRESSION OF THE CARDIAC NA+-CA2+ EXCHANGER IN END-STAGE HUMAN HEART-FAILURE
GENE-EXPRESSION OF THE CARDIAC NA+-CA2+ EXCHANGER IN END-STAGE HUMAN HEART-FAILURE
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DOI：
10.1161/01.res.75.3.443
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发表时间：
1994-09-01
影响因子：
20.1
通讯作者：
DREXLER, H
中科院分区：
文献类型：
--
作者：
STUDER, R;REINECKE, H;DREXLER, H
在衰竭的人心脏中，兴奋-收缩偶联过程中细胞内钙离子浓度的调节发生了变化。以前的研究主要集中在肌浆网(SR)对Cazt释放和重摄取的干扰，而对于衰竭的心脏，缺乏对心肌细胞内稳态的另一个重要决定因素--心肌Na+-Ca~(2+)交换的功能研究。利用最近从豚鼠c DNA文库中克隆的心肌Na+-Ca~(2+)交换器基因，研究了心肌Na~+-Ca~(2+)交换器与肌浆网Ca~(2+)-ATPase的基因表达。通过狭缝印迹分析，定量分析了24例失败的人心脏外植体和7例对照心脏标本的左室心肌中这两个基因的表达与β-肌球蛋白重链mRNA的关系。扩张型心肌病(DCM，n=13)患者Na+-Ca~(2+)交换器基因表达水平较非心力衰竭患者升高55%(P
The regulation of cytosolic Ca2+ concentration during excitation-contraction coupling is altered in the failing human heart. Previous studies have focused on disturbances in Cazt release and reuptake from the sarcoplasmic reticulum (SR), whereas functional studies of the cardiac Na+-Ca2+ exchanger, another important determinant of myocyte homeostasis, are lacking for the failing human heart. Using a cardiac Na+-Ca2+ exchanger cDNA recently cloned from a guinea pig cDNA library, we investigated the gene expression of the cardiac Na+-Ca2+ exchanger in relation to the SR Ca2+ ATPase. Expression of both genes was quantified in left ventricular myocardium from 24 failing human cardiac explants and 7 control heart samples in relation to beta-myosin heavy chain mRNA by slot blot analysis. Compared with patients with nonfailing hearts, patients with dilated cardiomyopathy (DCM, n=13) showed a 55% increase in Na+-Ca2+ exchanger mRNA levels (P