Optimization of an Analytical Procedure for Extraction of Lipids from Microalgae
Optimization of an Analytical Procedure for Extraction of Lipids from Microalgae
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DOI：
10.1007/s11746-011-1903-z
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发表时间：
2012-02-01
影响因子：
2
通讯作者：
Foubert, Imogen
中科院分区：
文献类型：
--
作者：
Ryckebosch, Eline;Muylaert, Koenraad;Foubert, Imogen
提出了一种从微藻中提取总脂和非极性脂的优化工艺。考察了溶剂、预处理（冻干、脂肪酶灭活和添加抗氧化剂）和细胞破碎（液氮、超声处理和珠击）对总脂质含量、脂质类和脂肪酸组成的影响。结果表明，氯仿-甲醇1：1是提取微藻总脂的最佳溶剂。当进行这种提取时，可以使用冻干的藻类，不需要用异丙醇预处理以使脂肪酶稳定，并且不需要添加抗氧化剂。此外，细胞破碎不是必需的，尽管在这种情况下必须连续进行两次提取以确保提取所有脂质，而不管微藻物种如何。非极性脂质含量的测定应通过在SPE柱上分离总脂质提取物来进行。仅当使用珠式搅拌器进行预处理时，才适合使用石油醚进行提取。
An optimized procedure for extraction of total and non-polar lipids from microalgae is proposed. The effects of solvent, pretreatment (lyophilization, inactivation of lipases, and addition of antioxidants) and cell-disruption (liquid nitrogen, sonication, and bead beating) on total lipid content, lipid class, and fatty acid composition were examined. Chloroform-methanol 1:1 was shown to be the best solvent mixture for extraction of total lipids from microalgae. When performing this extraction, lyophilized algae can be used, no pretreatment with isopropanol to inactivate the lipases is needed and addition of antioxidants is not necessary. Furthermore, cell-disruption is not essential, although in that case two extractions must be performed in series to ensure that, irrespective of the microalgal species, all lipids are extracted. Determination of non-polar lipid content should be performed by separation of the total lipid extract on an SPE column. Extraction using petroleum ether is only appropriate when a bead beater is used for pretreatment.