Blood-aggregating hydrogel particles for use as a hemostatic agent
Blood-aggregating hydrogel particles for use as a hemostatic agent
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DOI：
10.1016/j.actbio.2013.10.029
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发表时间：
2014-02-01
影响因子：
9.7
通讯作者：
Kofinas, Peter
中科院分区：
文献类型：
--
作者：
Behrens, Adam M.;Sikorski, Michael J.;Kofinas, Peter
身体无法控制大量失血而不进行治疗。可用的止血剂通常昂贵、无效或引起安全性问题。合成水凝胶颗粒是一种廉价且有前途的替代品。在这项研究中，我们合成和表征N-（3-氨丙基）甲基丙烯酰胺（APM）水凝胶颗粒，并研究其作为止血材料的用途。采用反相悬浮聚合法合成了粒径分布窄、溶胀快的APM水凝胶粒子。体外凝血研究显示水凝胶颗粒血液聚集体形成以及大量血液凝固抑制。使用多个大鼠损伤和绵羊肝撕裂模型的体内研究证明了颗粒有助于快速止血的能力。随后对绵羊肝脏切口部位进行苏木精和伊红染色和卡斯泰尔斯法染色，结果显示有显著的止血栓形成。该研究表明，这些阳离子水凝胶颗粒通过形成聚集体形成对失血的物理屏障，同时引起本体中凝血活性的普遍降低。通过聚集形成快速封闭剂和通过静电相互作用促进局部止血，同时降低整体凝血活性。这些相互作用需要来自简单合成平台的各种机制的相互作用。(C)2013 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
The body is unable to control massive blood loss without treatment. Available hemostatic agents are often expensive, ineffective or raise safety concerns. Synthetic hydrogel particles are an inexpensive and promising alternative. In this study we synthesized and characterized N-(3-aminopropyl)methacrylamide (APM) hydrogel particles and investigated their use as a hemostatic material. The APM hydrogel particles were synthesized via inverse suspension polymerization with a narrow size distribution and rapid swelling behavior. In vitro coagulation studies showed hydrogel particle blood aggregate formation as well as bulk blood coagulation inhibition. In vivo studies using multiple rat injury and ovine liver laceration models demonstrated the particles' ability to aid in rapid hemostasis. Subsequent hematoxylin and eosin and Carstairs' method staining of the ovine liver incision sites showed significant hemostatic plug formation. This study suggests that these cationic hydrogel particles form a physical barrier to blood loss by forming aggregates, while causing a general decrease in coagulation activity in the bulk. The formation of a rapid sealant through aggregation and the promotion of local hemostasis through electrostatic interactions are coupled with a decrease in overall coagulation activity. These interactions require the interplay of a variety of mechanisms stemming from a simple synthetic platform. (C) 2013 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.