CYSTEINE AND GLUTATHIONE CONCENTRATIONS IN PLASMA AND BRONCHOALVEOLAR LAVAGE FLUID AFTER TREATMENT WITH N-ACETYLCYSTEINE
CYSTEINE AND GLUTATHIONE CONCENTRATIONS IN PLASMA AND BRONCHOALVEOLAR LAVAGE FLUID AFTER TREATMENT WITH N-ACETYLCYSTEINE
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DOI：
10.1136/thx.46.1.39
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发表时间：
1991-01-01
期刊：
影响因子：
10
通讯作者：
RYLE, AP
中科院分区：
文献类型：
--
作者：
BRIDGEMAN, MME;MARSDEN, M;RYLE, AP
在支气管镜检查和支气管肺泡灌洗前，患者口服N-乙酰半胱氨酸（600 mg/天）5天，以确定N-乙酰半胱氨酸是否能增加血浆和支气管肺泡灌洗液中抗氧化剂还原型谷胱甘肽的浓度。在最后一剂N-乙酰半胱氨酸后1-3小时（组2，n = 9）和16-20小时（组3，n = 10）进行支气管肺泡灌洗，并将这些值与未接受N-乙酰半胱氨酸的对照组的值进行比较，在血浆或灌洗液中未检测到N-乙酰半胱氨酸。 接受治疗的组（组2和组3）中半胱氨酸（N-乙酰半胱氨酸的主要代谢产物和还原型谷胱甘肽的前体）的血浆浓度高于组1。 三组灌洗液中半胱氨酸浓度相似。 第2组血浆和灌洗液中还原型谷胱甘肽的浓度均高于第1组。 这些数据表明，经口给予的N-乙酰半胱氨酸迅速脱乙酰化为半胱氨酸，导致血浆中半胱氨酸浓度和血浆和气道中还原型谷胱甘肽浓度增加，从而暂时增加肺的抗氧化能力。
N-acetylcysteine (600 mg/day) was given to patients by mouth for five days before bronchoscopy and bronchoalveolar lavage to determine whether N-acetylcysteine could increase the concentrations of the antioxidant reduced glutathione in plasma and bronchoalveolar lavage fluid. Bronchoalveolar lavage was performed 1-3 hours (group 2, n = 9) and 16-20 hours (group 3, n = 10) after the last dose of N-acetylcysteine and the values were compared with those in a control group receiving no N-acetylcysteine was not detected in plasma or lavage fluid. Plasma concentrations of cysteine, the main metabolite of N-acetylcysteine and a precursor of reduced glutathione, were greater in the groups receiving treatment (groups 2 and 3) than in group 1. Cysteine concentrations in lavage fluid were similar in the three groups.. concentrations of reduced glutathione were greater in both plasma and lavage fluid in group 2 than in group 1. These data suggest that N-acetylcysteine given by mouth is rapidly deacetylated to cysteine, with resulting increases in the concentrations of cysteine in plasma and of reduced glutathione in plasma and the airways, which thus temporarily increase the antioxidant capacity of the lung.