Synthesis of a 2'-Se-thymidine phosphoramidite and its incorporation into oligonucleotides for crystal structure study.
Synthesis of a 2'-Se-thymidine phosphoramidite and its incorporation into oligonucleotides for crystal structure study.
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DOI：
10.1021/ol062937w
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发表时间：
2007-01
期刊：
影响因子：
5.2
通讯作者：
J. Sheng;Jiansheng Jiang;J. Saloň;Zhen Huang
中科院分区：
文献类型：
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作者：
J. Sheng;Jiansheng Jiang;J. Saloň;Zhen Huang
[反应：见正文] 为了研究用于晶体学的硒衍生化核酸，我们报告了 2'-甲基硒代胸苷亚磷酰胺的首次合成，并通过固相合成将其掺入 DNA 和 RNA，偶联产率超过 99%。还使用 Se 定相在 1.40 A 分辨率下测定了 d(GT(Se)GTACAC)2 晶体结构，表明这种 Se 衍生化不会引起显着的结构扰动，这与我们的 UV 熔化研究一致。此外，我们观察到 Se 改性很大程度上促进了结晶。
[reaction: see text] To investigate nucleic acids with selenium derivatization for crystallography, we report the first synthesis of 2'-methylseleno-thymidine phosphoramidite and its incorporation into DNAs and RNAs by solid-phase synthesis with over 99% coupling yield. The d(GT(Se)GTACAC)2 crystal structure was also determined at 1.40 A resolution using Se phasing, revealing that this Se derivatization did not cause significant structure perturbation, consistent with our UV melting study. In addition, we observed that the Se modification largely facilitated the crystallization.