A comparison of two exposure systems to apply malathion to Lumbricus terrestris L
A comparison of two exposure systems to apply malathion to Lumbricus terrestris L
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DOI：
10.1007/s00128-007-9194-7
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发表时间：
2007-06-01
影响因子：
2.7
通讯作者：
Stoskopf, M. K.
中科院分区：
文献类型：
--
作者：
Henson-Ramsey, H.;Kennedy-Stoskopf, S.;Stoskopf, M. K.
材料和方法将蚯蚓（Lumbricusterrestris）（Marblehead，俄亥俄州的国家饵料和钓具供应协会（NationalAssociationofSupplyofBaitandTackleofMarblehead，Ohio））在10 ℃的冰箱中在商业土壤产品基质（Scott ′ sgardensoil）上保持在聚乙烯盒（32 cm × 13.5 cm）中。将蚯蚓以60只蠕虫/容器的密度储存在用100 ml去离子水润湿的900 g土壤中。用湿润的粗棉布和铝箔覆盖这些盒子，用18号针头穿孔24次以进行空气流通。每隔一周向每个容器中加入30克兔子粪便以喂养蠕虫。仅使用具有发育良好的阴蒂的蠕虫进行测定。
Materials and MethodsLumbricus terrestris (National Association of Supplies Bait and Tackle of Marblehead, Ohio) were maintained in polyethylene boxes (32 cm by 13.5 cm) at 10 C in a refrigerator on a commercial soil product substrate (Scott’s® garden soil). The earthworms were stocked at a density of 60 worms/container in 900 g of soil moistened with 100 ml of deionized water. The boxes were covered with moistened cheesecloth and aluminum foil perforated 24 times with an 18-gauge needle for air circulation. Thirty grams of rabbit feces was added to each container every other week to feed the worms. Only adult worms with well-developed clitellum were used for the assays.