Roles of hepatocyte growth factor and its receptor in gastric mucosa
Roles of hepatocyte growth factor and its receptor in gastric mucosa
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肝细胞生长因子及其受体在胃粘膜中的作用
DOI：
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发表时间：
1995
影响因子：
3.1
通讯作者：
T. Kamada
中科院分区：
文献类型：
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作者：
S. Tsuji;S. Kawano;M. Tsujii;H. Fusamoto;T. Kamada
肝细胞生长因子（HGF）刺激肝细胞和其他上皮细胞的生长。在肠道、肝脏以及胃癌细胞中检测到 HGF 受体基因 c-met。然而，HGF在正常胃粘膜再生中的作用尚不清楚。本研究的目的是阐明HGF对培养胃粘膜细胞形态发生的影响，并评估HGF和c-met在大鼠胃粘膜愈合过程中的作用。培养的胃粘膜细胞在补充有 HGF 或胎牛血清的胶原基质中形成分支形态。它们不会在塑料皿上或在没有 HGF 或血清的胶原蛋白中形成这种形态。在一项体内研究中，在暴露于 0.6 M HCl 溶液后 6、24、48 和 96 小时从大鼠胃粘膜中提取总 RNA。未检测到HGF信使RNA，但粘膜中表达butc-met。 c-met表达增加后粘膜损伤愈合。这些结果表明HGF在胃粘膜细胞的形态发生中起重要作用，并且HGF受体基因参与胃粘膜的愈合过程。
Hepatocyte growth factor (HGF) stimulates the growth of hepatocytes and other epithelial cells. A gene for the HGF receptor,c-met, is detected in the intestinal tract and the liver, as well as in gastric carcinoma cells. However, the role of HGF in the regeneration of the normal gastric mucosa is not known. The purpose of the present study was to elucidate the effects of HGF on the morphogenesis of cultured gastric mucosal cells and to evaluate the role of HGF andc-met in the healing process in rat gastric mucosa. The cultured gastric mucosal cells developed a branching morphology in a collagen matrix supplemented with HGF or fetal calf serum. They did not form this morphology on a plastic dish or in the collagen without HGF or the serum. In anin vivo study, total RNA was extracted from rat gastric mucosa 6, 24, 48, and 96 hr after the exposure to a solution of 0.6 M HCl. HGF messenger RNA was not detected, butc-met was expressed in the mucosa. The increased expression ofc-met was followed by healing of the mucosal injury. These results indicate that HGF plays important roles in the morphogenesis of gastric mucosal cells and that the HGF receptor gene participates in the healing process of gastric mucosa.