Isolation, culture, and transplantation of human hepatocytes.
Isolation, culture, and transplantation of human hepatocytes.
复制标题
DOI：
10.1093/jnci/68.5.771
复制
发表时间：
1982-05
期刊：
影响因子：
--
通讯作者：
S. Strom;R. Jirtle;R. S. Jones;D. Novicki;M. R. Rosenberg;A. Novotny;G. Irons;J. R. McLain;G. Michalopoulos
中科院分区：
文献类型：
--
作者：
S. Strom;R. Jirtle;R. S. Jones;D. Novicki;M. R. Rosenberg;A. Novotny;G. Irons;J. R. McLain;G. Michalopoulos
用于从大鼠肝脏中分离肝细胞的原位两步胶原酶灌注技术被改编为适用于从外科手术中获得的人肝脏块的程序。用这种方法获得的人肝细胞在原代培养中保存10天。描述了在光镜和电子显微镜水平上观察到的细胞变化。微粒体酶的变化作为培养年龄的函数也被测量。人类肝细胞暴露于已知由啮齿动物肝脏代谢的致癌物原导致计划外的DNA合成。分离的肝细胞也被移植到三分之二的部分肝切除的胸腺裸鼠体内。移植细胞形成结节，具有典型的肝脏结构。这些研究表明，通过所描述的改良胶原酶技术从人肝脏中获得的肝细胞可用于体外化学致癌研究。
The in situ two-step collagenase perfusion technique used for the isolation of hepatocytes from rat liver was adapted into a procedure applicable to pieces of human liver obtainable from surgical procedures. Human hepatocytes obtained by this method were maintained in primary culture for 10 days. The cellular changes observed at the light microscopic and electron microscopic levels are described. The changes in microsomal enzymes as a function of the age of the cultures were also measured. Exposure of the human hepatocytes to procarcinogens known to be metabolized by rodent liver resulted in unscheduled DNA synthesis. The isolated hepatocytes were also transplanted into two-thirds partially hepatectomized athymic nude mice. The transplanted cells formed nodules with characteristic hepatic architecture. These studies demonstrate that hepatocytes obtained from human liver by the described modified collagenase technique can be used for in vitro studies in chemical carcinogenesis.