Novel implantable imaging system for enabling simultaneous multiplanar and multipoint analysis for fluorescence potentiometry in the visual cortex.
Novel implantable imaging system for enabling simultaneous multiplanar and multipoint analysis for fluorescence potentiometry in the visual cortex.
复制标题
新型植入式成像系统，可对视觉皮层中的荧光电位进行同步多平面和多点分析。
DOI：
10.1016/j.bios.2012.06.035
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发表时间：
2012
影响因子：
12.6
通讯作者：
(他11名)
中科院分区：
文献类型：
--
作者：
Kobayashi T;(他11名)
在大范围内快速、无创地测量神经元兴奋性的技术对于分析和理解神经元网络和动物行为具有重要意义。在这项研究中，一种新的植入式成像系统的荧光电位法开发使用互补金属氧化物半导体（CMOS）技术，并将其应用于培养的脑切片和活体小鼠的大脑的分析。开发了一种CMOS图像传感器，它足够小，可以植入大脑，带有发光二极管和吸收滤光片，可以实现实时荧光成像。该传感器结合电压敏感染料，当然能够可视化神经元的潜在状态，并通过首次使用多个传感器同时获得左右视觉皮层的生理反应。这种多平面和多点测量的完成提供了多维信息，从不同的方面。光微传感器不会干扰动物的行为。这意味着成像系统可以将大脑中的功能性荧光成像与自由移动动物的行为实验相结合。
Techniques for fast, noninvasive measurement of neuronal excitability within a broad area will be of major importance for analyzing and understanding neuronal networks and animal behavior in neuroscience field. In this research, a novel implantable imaging system for fluorescence potentiometry was developed using a complementary metal-oxide semiconductor (CMOS) technology, and its application to the analysis of cultured brain slices and the brain of a living mouse is described. A CMOS image sensor, small enough to be implanted into the brain, with light-emitting diodes and an absorbing filter was developed to enable real-time fluorescence imaging. The sensor, in conjunction with a voltage-sensitive dye, was certainly able to visualize the potential statuses of neurons and obtain physiological responses in both right and left visual cortex simultaneously by using multiple sensors for the first time. This accomplished multiplanar and multipoint measurement provides multidimensional information from different aspects. The light microsensors do not disturb the animal behavior. This implies that the imaging system can combine functional fluorescence imaging in the brain with behavioral experiments in a freely moving animal.