Effect of Pseudomonas sp. HF-1 inoculum on construction of a bioaugmented system for tobacco wastewater treatment: analysis from quorum sensing
Effect of Pseudomonas sp. HF-1 inoculum on construction of a bioaugmented system for tobacco wastewater treatment: analysis from quorum sensing
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DOI：
10.1007/s11356-014-2760-8
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发表时间：
2014-03
影响因子：
5.8
通讯作者：
Meizhen Wang;Hong-zhen He;Xin Zheng;Huajun Feng;Z. Lv;D. Shen
中科院分区：
文献类型：
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作者：
Meizhen Wang;Hong-zhen He;Xin Zheng;Huajun Feng;Z. Lv;D. Shen
为更好地构建生物强化系统处理烟草废水，将不同浓度的烟碱降解菌Pseudomonassp接种到活性污泥中。HF-1结果表明，接种量为0.55 ± 0.01和1.10 ± 0.03 mg/g（HF-1干重/活性污泥干重）时，HF-1的存活率和生物强化效果最好。研究了生物强化系统中群体感应自诱导物（AI）的释放规律。在HF-1接种期间，与失败的生物强化系统相比，成功的系统中AI-2显著增加，表明AI-2介导的细菌通讯在HF-1的定殖中起重要作用。当停止菌株HF-1的接种时，AI-2的量在所有系统中下降并趋于稳定。值得注意的是，在失败的生物强化系统中，短链AHLs增加了三倍以上，但在成功的系统中没有增加，这意味着短链AHLs的波动可能是生物强化失败的指标。因此，AI-2介导的群体感应可以实现以促进HF-1定殖。
To better construct a bioaugmented system for tobacco wastewater treatment, activated sludge was inoculated with different concentrations of the nicotine-degrading bacteriumPseudomonassp. HF-1. The results showed that inoculum concentrations of 0.55 ± 0.01 and 1.10 ± 0.03 mg/g (dry weight of strain HF-1/dry weight of activated sludge) were best to ensure strain HF-1 survival and successful bioaugmentation. The release pattern of autoinducer (AI) for quorum sensing in the bioaugmented system was also investigated. During the period of HF-1 inoculation, compared with failed bioaugmented systems, AI-2 was significantly increased in the successful systems, suggesting that AI-2-mediated bacterial communication played an important role in the colonization of HF-1. When inoculation of strain HF-1 was stopped, the amount of AI-2 decreased and leveled out in all systems. Notably, there was a greater than threefold increase of short-chain AHLs in failed bioaugmented systems, but no increase in successful ones, implying that the fluctuation of short-chain AHLs could be an indicator of the failure of bioaugmentation. Thus, AI-2-mediated quorum sensing could be implemented to facilitate HF-1 colonization.